Rizzino A, Mastanduno M, Freundlich M
Biochim Biophys Acta. 1977 Mar 18;475(2):267-75. doi: 10.1016/0005-2787(77)90017-x.
Methionine starvation of methionine auxotrophs in the presence of excess branched-chain amino acids results in a partial derepression of the isoleucine and valine enzymes. Reversed-phase chromatography indicated that isoleucine, valine and leucine tRNA were altered during methionine starvation. In addition, the total tRNA isolated from cells under these conditions were undermethylated. The observed derepression may be caused by the inability of methyl-deficient tRNA's to participate adequately in normal regulatory functions.
在存在过量支链氨基酸的情况下，对甲硫氨酸营养缺陷型进行甲硫氨酸饥饿处理会导致异亮氨酸和缬氨酸酶的部分去阻遏。反相色谱法表明，在甲硫氨酸饥饿期间，异亮氨酸、缬氨酸和亮氨酸的tRNA发生了改变。此外，在这些条件下从细胞中分离出的总tRNA甲基化不足。观察到的去阻遏可能是由于甲基缺乏的tRNA无法充分参与正常调节功能所致。
