甲萘醌和还原型甲萘醌对噬菌体φX174 DNA感染性的灭活作用

Inactivation of infectivity of phiX174 DNA by menadione and reduced menadione.

作者信息

Morita J, Narita S, Komano T

出版信息

Biochim Biophys Acta. 1977 Mar 18;475(2):403-7. doi: 10.1016/0005-2787(77)90029-6.

Abstract

Interaction of menadione and reduced menadione with phage phiX174 DNA was investigated. A concentration of 2-10(-4) M menadione inactivated 60% of the infectivity of phiX174 DNA to spheroplasts of Escherichia coli, while reduced menadione inactivated 97% of the infectivity of phiX174 DNA at the same concentration. Alkaline sucrose gradient centrifugation revealed 2-10(-5) M reduced menadione caused approximately 24% of phiX174 DNA to produce strand break under the condition of 80% lethanlity. DNA strand break was not observed even at 4 - 10(-4) M menadione. These results indicated that there were different mechanisms for inactivation of phiX174 DNA between menadione and reduced menadione.

摘要

研究了甲萘醌和还原型甲萘醌与噬菌体φX174 DNA的相互作用。浓度为2×10⁻⁴ M的甲萘醌使φX174 DNA对大肠杆菌原生质球的感染力失活60%，而相同浓度的还原型甲萘醌使φX174 DNA的感染力失活97%。碱性蔗糖梯度离心显示，在80%致死率的条件下，2×10⁻⁵ M的还原型甲萘醌使约24%的φX174 DNA产生链断裂。即使在4×10⁻⁴ M的甲萘醌浓度下也未观察到DNA链断裂。这些结果表明，甲萘醌和还原型甲萘醌对φX174 DNA失活的机制不同。