Biliary Surgical Department of West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Chem Commun (Camb). 2020 Mar 25;56(24):3536-3538. doi: 10.1039/d0cc00397b. Epub 2020 Feb 27.
Herein, we introduce a sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5'-TTN PAM sequence can be recognized by Cas12a through a specific CRISPR RNA. The signal transduction is achieved using two orthogonal plasmonic systems mediated by Cas12a.
在这里，我们介绍了一种序列特异性的等离子体环介导等温扩增（LAMP）分析方法，该方法具有由 CRISPR Cas12a 实现的双重互补颜色读出功能。使用该测定法，任何含有 5'-TTN PAM 序列的双链 LAMP 扩增子都可以通过 Cas12a 通过特定的 CRISPR RNA 进行识别。信号转导是通过 Cas12a 介导的两个正交等离子体系统来实现的。
