一种基于 CRISPR Cas12a 的序列特异性等离子体环介导等温扩增分析方法，具有正交颜色读取功能。
A sequence-specific plasmonic loop-mediated isothermal amplification assay with orthogonal color readouts enabled by CRISPR Cas12a.
机构信息
Biliary Surgical Department of West China Hospital, Sichuan University, Chengdu, Sichuan, China.
出版信息
Chem Commun (Camb). 2020 Mar 25;56(24):3536-3538. doi: 10.1039/d0cc00397b. Epub 2020 Feb 27.
Herein, we introduce a sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5'-TTN PAM sequence can be recognized by Cas12a through a specific CRISPR RNA. The signal transduction is achieved using two orthogonal plasmonic systems mediated by Cas12a.
在这里，我们介绍了一种序列特异性的等离子体环介导等温扩增（LAMP）分析方法，该方法具有由 CRISPR Cas12a 实现的双重互补颜色读出功能。使用该测定法，任何含有 5'-TTN PAM 序列的双链 LAMP 扩增子都可以通过 Cas12a 通过特定的 CRISPR RNA 进行识别。信号转导是通过 Cas12a 介导的两个正交等离子体系统来实现的。
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