Department of Bioengineering, Imperial College London, London, United
Eur Cell Mater. 2020 Feb 27;39:136-155. doi: 10.22203/eCM.v039a09.
The establishment of a complex collagen network is critical for the architecture and mechanical properties of cartilage and bone. However, when and how the key collagens in cartilage and bone develop has not been characterised in detail. The study provides a detailed qualitative characterisation of the spatial localisations of collagens I-III, V-VI and IX-XI in the mouse and their regional architecture variation over three developmentally significant time points: when the rudiment starts to form at E13.5 [Theiler stage (TS) 22], when mineralisation is present at E16.5 (TS25) and during the latest prenatal stage at E18.5 (TS27). Dynamic changes in collagen distribution between stages with the progression of the growth plate and mineralisation (particularly collagens I, II, V, X and XI) and dramatic changes in collagen structural organisation and complexity with maturation, especially for collagens II and XI, were observed. The future articular cartilage region was demarcated by pronounced collagens II and VI expression at TS27 and the emergence of collagens I, III, V, IX and XI in the tendon and its insertion site was observed. The present study revealed, for the first time, the emergence and maturation of key cartilage and bone collagens, in high resolution, at multiple locations across the entire rudiment, including the joint regions, at three of the most developmentally significant stages of skeletogenesis, furthering the understanding of disease and regeneration of skeletal tissues.
建立一个复杂的胶原网络对于软骨和骨骼的结构和机械性能至关重要。然而，软骨和骨骼中的关键胶原何时以及如何发育还没有详细描述。本研究详细定性地描述了在小鼠中 I-III、V-VI 和 IX-XI 型胶原在三个发育重要时间点的空间定位及其区域结构变化：当胚胎在 E13.5 开始形成时（Theiler 期 22），当 E16.5 时出现矿化时（Theiler 期 25）以及在 E18.5 的最晚产前阶段（Theiler 期 27）。随着生长板和矿化的进展，胶原分布在各阶段之间发生动态变化（特别是 I、II、V、X 和 XI 型胶原），并且随着成熟，胶原结构组织和复杂性发生剧烈变化，特别是对于 II 和 XI 型胶原。在 TS27 时，未来的关节软骨区域被明显的 II 型和 VI 型胶原标记，并且在肌腱及其插入部位观察到 I、III、V、IX 和 XI 型胶原的出现。本研究首次在骨骼发生的三个最具发育意义的阶段之一的整个胚胎的多个部位，包括关节区域，以高分辨率揭示了关键软骨和骨骼胶原的出现和成熟，这有助于理解骨骼组织的疾病和再生。
