Karimi-Yazdi Mohammadmahdi, Ghalavand Zohreh, Shabani Mahdi, Houri Hamidreza, Sadredinamin Mehrzad, Taheri Marzieh, Eslami Gita
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Infect Drug Resist. 2020 Feb 13;13:485-492. doi: 10.2147/IDR.S238559. eCollection 2020.
continues to be important causes of acute pediatric diarrhea worldwide. produces numerous virulence factors involved in colonization and invasion into epithelial cells which eventually result in the disease. The present study was conducted to evaluate the prevalence of virulence genes and to investigate antibiotic resistance profiles among isolates obtained from pediatric patients in Iran.
A total of 141 isolates were collected between March 2017 and September 2018 from stool of children under 14 who were suspected to have shigellosis. isolates were identified using standard microbiological and serological tests and antimicrobial susceptibility testing was carried out via Kirby-Bauer disk diffusion method. In addition, the presence of seven virulence determinants including , and were evaluated using PCR.
(78.7%) was the most prevalent spp. among children with shigellosis followed by (19.9%) and (1.4%). Antimicrobial susceptibility testing revealed that most of the isolates were considered as multidrug-resistant (MDR) strains. Our findings also showed a high resistance rate against trimethoprim-sulfamethoxazole in isolates. The prevalence of , and were 100%, 95.7%, 95.7%, 94.3%, 93.6%, 92.9%, and 80.8%, respectively.
The current study revealed that was the predominant species isolated from children with shigellosis in Iran. Our results also indicated a high distribution of type III secretion system effector protein-encoding genes and high multidrug-resistance among shigella spp. in Iran. Therefore, it is suggested that antimicrobial susceptibility testing be performed prior to antibiotic prescription.
在全球范围内，仍是小儿急性腹泻的重要病因。它产生许多与定植和侵入上皮细胞有关的毒力因子，最终导致疾病。本研究旨在评估毒力基因的流行情况，并调查从伊朗儿科患者中分离出的菌株的抗生素耐药性谱。
2017年3月至2018年9月期间，共从14岁以下疑似患有志贺氏菌病的儿童粪便中收集了141株菌株。通过标准微生物学和血清学试验鉴定菌株，并采用 Kirby-Bauer 纸片扩散法进行药敏试验。此外，利用聚合酶链反应评估包括、和在内的7种毒力决定因素的存在情况。
（78.7%）是志贺氏菌病患儿中最常见的志贺氏菌属，其次是（19.9%）和（1.4%）。药敏试验显示，大多数分离株被认为是多重耐药（MDR）菌株。我们的研究结果还表明，志贺氏菌分离株对甲氧苄啶-磺胺甲恶唑的耐药率很高。、和的流行率分别为100%、95.7%、95.7%、94.3%、93.6%、92.9%和80.8%。
目前的研究表明，是伊朗从志贺氏菌病患儿中分离出的主要菌种。我们的结果还表明，伊朗志贺氏菌属中III型分泌系统效应蛋白编码基因的分布很高，且多重耐药性也很高。因此，建议在开抗生素处方前进行药敏试验。
