Travnickova Martina, Pajorova Julia, Zarubova Jana, Krocilova Nikola, Molitor Martin, Bacakova Lucie
Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech Republic.
Second Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague 5, Czech Republic.
Stem Cells Int. 2020 Feb 10;2020:1016231. doi: 10.1155/2020/1016231. eCollection 2020.
Adipose tissue-derived stromal cells (ADSCs) have great potential for cell-based therapies, including tissue engineering. However, various factors can influence the characteristics of isolated ADSCs.
We studied the influence of the harvesting site, i.e., inner thigh ( = 3), outer thigh ( = 3), outer thigh ( = 3), outer thigh (.
We revealed higher initial cell yields from the outer thigh region than from the abdomen region. Negative pressure did not influence the cell yields from the outer thigh region, whereas the yields from the abdomen region were higher under high negative pressure than under low negative pressure. In the subsequent passage, in general, no significant relationship was identified between the different negative pressure and ADSC characteristics. No significant difference was observed in the characteristics of thigh ADSCs and abdomen ADSCs. Only on day 1, the diameter was significantly bigger in outer thigh ADSCs than in abdomen ADSCs. Moreover, we noted a tendency of thigh ADSCs (i.e., inner thigh+outer thigh) to reach a higher cell number on day 7. . The harvesting site and negative pressure can potentially influence initial cell yields from lipoaspirates. However, for subsequent culturing and for use in tissue engineering, it seems that the harvesting site and the level of negative pressure do not have a crucial or limiting effect on basic ADSC characteristics. culturing and for use in tissue engineering, it seems that the harvesting site and the level of negative pressure do not have a crucial or limiting effect on basic ADSC characteristics.
脂肪组织来源的基质细胞（ADSCs）在包括组织工程在内的基于细胞的治疗中具有巨大潜力。然而，多种因素会影响分离得到的ADSCs的特性。
我们研究了取材部位的影响，即大腿内侧（=3）、大腿外侧（=3）、大腿外侧（=3）、大腿外侧（。
我们发现大腿外侧区域的初始细胞产量高于腹部区域。负压对大腿外侧区域的细胞产量没有影响，而腹部区域在高负压下的产量高于低负压下的产量。在后续传代中，一般来说，不同负压与ADSC特性之间未发现显著关系。大腿ADSCs和腹部ADSCs的特性没有显著差异。仅在第1天，大腿外侧ADSCs的直径明显大于腹部ADSCs。此外，我们注意到大腿ADSCs（即大腿内侧+大腿外侧）在第7天有达到更高细胞数量的趋势。取材部位和负压可能会影响抽脂物的初始细胞产量。然而，对于后续培养以及用于组织工程，取材部位和负压水平似乎对ADSCs的基本特性没有关键或限制作用。对于后续培养以及用于组织工程，取材部位和负压水平似乎对ADSCs的基本特性没有关键或限制作用。
