Dai Huizhen, Zhang Huan, Zu Xinxia, Wang Hongyan, Wang Lili
Department of Emergency, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, P.R. China.
Department of Ophthalmology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, P.R. China.
Exp Ther Med. 2020 Mar;19(3):1961-1968. doi: 10.3892/etm.2020.8409. Epub 2020 Jan 2.
The aims of the present study were to examine the clinical significance of serum microRNA-27a (miR-27a) levels in patients with multiple organ dysfunction syndrome (MODS) caused by acute paraquat poisoning and to investigate the correlation between miR-27a and interleukin (IL)-10. A total of 82 patients with MODS induced by acute paraquat poisoning and 88 healthy controls were recruited in the present study. Reverse transcription-quantitative PCR was used to measure serum miR-27a expression levels in patients with MODS and the control group. IL-10 serum levels were determined using ELISA. Decreased serum miR-27a level and increased IL-10 expression levels were detected in patients with paraquat poisoning compared with healthy controls (P<0.001). A moderately negative correlation was identified between the serum expression levels of miR-27a and IL-10 (r=-0.5225; P<0.001). miR-27a expression level was found to be associated with blood urea nitrogen, partial pressure of carbon dioxide, arterial blood lactic acid, and the acute physiology and chronic health evaluation II score (APACHE II; P<0.05). The area under the curve for miR-27a was 0.946, with a sensitivity of 86.6% and specificity of 87.5% at a cutoff value of 2.10. The non-survival patient group had lower miR-27a expression levels compared with the survival group (P<0.01). Multivariate Cox regression analyses suggested miR-27a expression level and APACHE II score were independent prognostic factors for 30-day mortality (P<0.01). The present results suggested that serum miR-27a level may be a potential novel diagnostic and prognostic factor for MODS caused by paraquat poisoning. Collectively, miR-27a may be involved in the process of MODS induced by paraquat poisoning by regulating the inflammatory response.
本研究的目的是探讨血清微小RNA-27a（miR-27a）水平在急性百草枯中毒所致多器官功能障碍综合征（MODS）患者中的临床意义，并研究miR-27a与白细胞介素（IL）-10之间的相关性。本研究共纳入82例急性百草枯中毒所致MODS患者和88例健康对照者。采用逆转录定量聚合酶链反应检测MODS患者和对照组血清miR-27a表达水平。采用酶联免疫吸附测定法测定血清IL-10水平。与健康对照者相比，百草枯中毒患者血清miR-27a水平降低，IL-10表达水平升高（P<0.001）。miR-27a与IL-10的血清表达水平之间存在中度负相关（r=-0.5225；P<0.001）。发现miR-27a表达水平与血尿素氮、二氧化碳分压、动脉血乳酸以及急性生理与慢性健康状况评分II（APACHE II）相关（P<0.05）。miR-27a的曲线下面积为0.946，在临界值为2.10时，灵敏度为86.6%，特异度为87.5%。与存活组相比，非存活患者组的miR-27a表达水平较低（P<0.01）。多因素Cox回归分析表明，miR-27a表达水平和APACHE II评分是30天死亡率的独立预后因素（P<0.01）。本研究结果表明，血清miR-27a水平可能是百草枯中毒所致MODS的一个潜在的新型诊断和预后因素。总体而言，miR-27a可能通过调节炎症反应参与百草枯中毒诱导的MODS过程。
