Emergency Department, 155177Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser hospital), Qingdao, Shandong, China.
Hum Exp Toxicol. 2022 Jan-Dec;41:9603271221094008. doi: 10.1177/09603271221094008.
Acute paraquat poisoning-induced multiple organ dysfunction syndrome (MODS) leads to the high mortality. This study aimed to investigate the clinical significance of microRNA-200b-3p (miR-200b-3p), an upstream inhibitor of high-mobility group box 1 (HMGB1), in acute paraquat poisoning patients for the prediction of MODS and survival.
This study enrolled 80 patients with MODS induced by paraquat and 94 healthy volunteers. The interaction between miR-200b-3p and HMGB1 was identified by luciferase reporter assay. miR-200b-3p levels were measured by quantitative real-time (QRT) PCR. High-mobility group box 1 levels were measured by enzyme-linked immune sorbent assay (ELISA). Receiver operating characteristic analysis was used to evaluate the diagnostic value of miR-200b-3p in screening MODS patients. The relationship between miR-200b-3p and the 28-day survival of MODS patients was evaluated by Kaplan-Meier curves and log-rank test. Cox regression analysis was used to assess the prognostic value of miR-200b-3p. Correlation between miR-200b-3p and HMGB1 was confirmed by Pearson's correlation analysis.
miR-200b-3p directly target HMGB1. miR-200b-3p, decreased in MODS patients, had high diagnostic value to screen MODS patients from healthy controls. Additionally, serum miR-200b-3p was decreased in non-survivors, and patients with low miR-200b-3p level had poor 28-day survival. Serum miR-200b-3p could independently predict the survival prognosis. Moreover, serum HMGB1 level was increased in MODS patients, and was negatively correlated with miR-200b-3p level.
Decreased miR-200b-3p may function as a biomarker for the diagnosis and survival prognosis of MODS patients, and miR-200b-3p may be involved in the progression of acute paraquat-induced MODS via regulating inflammatory responses by targeting HMGB1.
急性百草枯中毒诱导的多器官功能障碍综合征（MODS）导致高死亡率。本研究旨在探讨高迁移率族蛋白 1（HMGB1）上游抑制剂微小 RNA-200b-3p（miR-200b-3p）在急性百草枯中毒患者中的临床意义，以预测 MODS 和生存。
本研究纳入了 80 例因百草枯引起的 MODS 患者和 94 名健康志愿者。通过荧光素酶报告实验鉴定 miR-200b-3p 与 HMGB1 之间的相互作用。采用实时定量聚合酶链反应（QRT-PCR）检测 miR-200b-3p 水平。采用酶联免疫吸附试验（ELISA）检测 HMGB1 水平。采用受试者工作特征分析评估 miR-200b-3p 筛查 MODS 患者的诊断价值。通过 Kaplan-Meier 曲线和对数秩检验评估 miR-200b-3p 与 MODS 患者 28 天生存率的关系。采用 Cox 回归分析评估 miR-200b-3p 的预后价值。采用 Pearson 相关分析证实 miR-200b-3p 与 HMGB1 的相关性。
miR-200b-3p 可直接靶向 HMGB1。在 MODS 患者中降低的 miR-200b-3p 对筛选来自健康对照的 MODS 患者具有较高的诊断价值。此外，非幸存者血清 miR-200b-3p 降低，低 miR-200b-3p 水平的患者 28 天生存率较差。血清 miR-200b-3p 可独立预测生存预后。此外，MODS 患者血清 HMGB1 水平升高，且与 miR-200b-3p 水平呈负相关。
降低的 miR-200b-3p 可能作为 MODS 患者诊断和生存预后的生物标志物，miR-200b-3p 可能通过靶向 HMGB1 调节炎症反应而参与急性百草枯诱导的 MODS 进展。
