Siregar Safendra, Adriansjah Ricky, Sibarani Jupiter, Mustafa Akhmad
Department of Urology, Faculty of Medicine, Universitas Padjadjaran, General Hospital Hasan Sadikin, Bandung, Indonesia.
Res Rep Urol. 2020 Feb 5;12:21-27. doi: 10.2147/RRU.S232303. eCollection 2020.
The ischemic process in priapism can lead to displacement of normal tissue with fibrotic tissue, due to collagen deposition, and eventually leads to erectile dysfunction. Many studies have identified that the supernatant of adipose tissue-derived stem cells (ADSCs) significantly ameliorates fibrosis of different tissue, but limited attention has been paid to its efficacy on fibrosis of the corpora cavernosa.
A total of 22 Wistar rats divided into five groups, with two groups each consisting of five male wistar rats with priapism without human ADSC (hADSC) therapy (group I) and two other groups consisting of five rats with priapism, were given 10 cells' intracorporeal hADSC injection after 12 hours of penile clamping (group II) were euthanized after 2 and 4 weeks of observation. The last group consisted of two rats without any treatment or model (group III). Following euthanasia, penises were harvested for TGFβ and collagen type I measurement using ELISA. Statistical analysis using independent-sample -tests was done with SPSS 21.0.
Penile TGFβ concentration in the treatment group was significantly lower in the second and fourth weeks of observation ( =0.004, =0.003), and collagen type I was significantly lower in the second and fourth weeks ( =0.003, =0.011).
Intracorporeal hADSC injection limited the fibrosis process in a priapism model. Although the mechanism was unclear, it may be related to the potential of hADSCs to produce various growth factors that could limit TGFβ and collagen production.
阴茎异常勃起中的缺血过程可导致正常组织被纤维化组织取代，这是由于胶原蛋白沉积所致，最终会导致勃起功能障碍。许多研究已证实，脂肪组织来源干细胞（ADSCs）的上清液能显著改善不同组织的纤维化，但对其在阴茎海绵体纤维化方面的疗效关注较少。
将22只Wistar大鼠分为五组，其中两组各有五只患有阴茎异常勃起且未接受人脂肪组织来源干细胞（hADSCs）治疗的雄性Wistar大鼠（I组），另外两组各有五只患有阴茎异常勃起的大鼠，在阴茎夹闭12小时后进行10个细胞的阴茎海绵体内hADSCs注射（II组），在观察2周和4周后实施安乐死。最后一组由两只未接受任何治疗或未建立模型的大鼠组成（III组）。安乐死后，取出阴茎，采用酶联免疫吸附测定法（ELISA）检测转化生长因子β（TGFβ）和I型胶原蛋白。使用SPSS 21.0软件进行独立样本t检验的统计分析。
在观察的第二周和第四周，治疗组阴茎TGFβ浓度显著降低（P = 0.004，P = 0.003），I型胶原蛋白在第二周和第四周也显著降低（P = 0.003，P = 0.011）。
阴茎海绵体内注射hADSCs可限制阴茎异常勃起模型中的纤维化过程。尽管其机制尚不清楚，但可能与hADSCs产生多种生长因子的能力有关，这些生长因子可限制TGFβ和胶原蛋白的产生。
