香烟烟雾水提取物的细胞毒性受用于制备提取物的移液管特性影响。
Cytotoxicity of aqueous cigarette smoke extract is affected by properties of pipettes used to prepare the extract.
作者信息
Bernstein Eric D, Liao Zhaohao, Witwer Kenneth W
机构信息
Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine.
出版信息
Matters (Zur). 2019;2019. Epub 2019 Oct 14.
Abstract
Cigarette smoke contains a host of molecules including toxins and carcinogens, most of which have not been well studied. Aqueous cigarette smoke extract (CSE) is one of various cigarette smoke derivatives that can be used for studies, and the influence of different method parameters on CSE composition and toxicity remains incompletely understood. Herein, we prepared CSE by bubbling cigarette smoke through mammalian cell culture medium, varying the type of pipette inserted into the recipient medium. Changing this one component of the preparation apparatus had a marked effect on the toxicity of the resulting CSE. Since many other parameters can also be varied in CSE preparation, these results stress the importance of standardization within and between studies.
摘要
香烟烟雾包含许多分子，包括毒素和致癌物，其中大多数尚未得到充分研究。香烟烟雾水提取物（CSE）是可用于研究的多种香烟烟雾衍生物之一，不同方法参数对CSE成分和毒性的影响仍未完全了解。在此，我们通过将香烟烟雾通入哺乳动物细胞培养基来制备CSE，改变插入受体培养基的移液管类型。改变制备装置的这一组成部分对所得CSE的毒性有显著影响。由于在CSE制备中许多其他参数也可以变化，这些结果强调了研究内部和研究之间标准化的重要性。
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