Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada.
Alberta Diabetes Institute, University of Alberta, Edmonton, AB, Canada.
PLoS One. 2018 Oct 16;13(10):e0204269. doi: 10.1371/journal.pone.0204269. eCollection 2018.
Mammalian cell culture is foundational to biomedical research, and the reproducibility of research findings across the sciences is drawing increasing attention. While many components contribute to reproducibility, the reporting of factors that impact oxygen delivery in the general biomedical literature has the potential for both significant impact, and immediate improvement. The relationship between the oxygen consumption rate of cells and the diffusive delivery of oxygen through the overlying medium layer means parameters such as medium depth and cell type can cause significant differences in oxygenation for cultures nominally maintained under the same conditions. While oxygenation levels are widely understood to significantly impact the phenotype of cultured cells in the abstract, in practise the importance of the above parameters does not appear to be well recognized in the non-specialist research community. On analyzing two hundred articles from high-impact journals we find a large majority missing at least one key piece of information necessary to ensure consistency in replication. We propose that explicitly reporting these values should be a requirement for publication.
哺乳动物细胞培养是生物医学研究的基础，科学界越来越关注研究结果的可重复性。虽然许多因素都有助于提高可重复性，但在一般的生物医学文献中报告影响氧气输送的因素具有重要的影响和直接的改进潜力。细胞的耗氧率与覆盖的介质层中氧气的扩散输送之间的关系意味着，介质深度和细胞类型等参数会导致在名义上相同条件下培养的细胞的氧合作用产生显著差异。虽然人们普遍认为氧合水平会显著影响培养细胞的表型，但实际上，在非专业研究领域，上述参数的重要性似乎并未得到很好的认识。在分析了来自高影响力期刊的 200 篇文章后，我们发现大多数文章至少缺少一条确保复制一致性所必需的关键信息。我们建议，明确报告这些值应该是发表的要求。
