Programa Moscafrut, Metapa de Domínguez, Chiapas, México.
Servicios Mubarqui S. de R. L. de C. V., Cuidad Victoria, Tamaulipas, México.
J Insect Sci. 2020 Jan 1;20(1). doi: 10.1093/jisesa/ieaa004.
Different densities prerelease packing and times of lethargy in the fruit fly parasitoids Diachasmimorpha longicaudata (Ashmead) were evaluated in order to standardize the process of chilled insect technique for this species. Adults were kept at densities of 0.048, 0.072, 0.096, 0.120, and 0.144 parasitoids/cm2 before release in a México tower, where thermal lethargy was induced at a temperature of 2 ± 2°C for 45 min. Samples of parasitoids were collected to evaluate mortality, survival, fecundity, and flight capacity. All densities showed a similar mortality, both for males (ca. >10%) and females (ca. <7). There was no effect of density on survival and flight capacity in both sexes. On the other hand, fecundity increased with density, 1.66 sons/♀/day, similar to the control. We conclude that a density of 30,000 pupae per cage (0.144 parasitoids/cm2) is adequate for the massive prerelease packaging of the parasitoid D. longicaudata. Regarding the thermal lethargy period, 180 min under 2 ± 2°C conditions, considered as time for management, does not affect the survival, fecundity, and flight capacity of adults. The results obtained are of great utility to establish prerelease packaging parameters for D. longicaudata used in the biological control of Tephritidae fruit fly populations.
为了使该物种的冷藏昆虫技术标准化，评估了异色瓢虫（Diachasmimorpha longicaudata（Ashmead））在预释放前的不同密度包装和休眠时间。成虫在墨西哥塔中以 0.048、0.072、0.096、0.120 和 0.144 个寄生蜂/cm2 的密度释放前进行饲养，在 2±2°C 的温度下诱导热休眠 45 分钟。收集寄生蜂样本以评估死亡率、存活率、繁殖力和飞行能力。所有密度下雄性（约>10%）和雌性（约<7%）的死亡率都相似。密度对雌雄两性的存活率和飞行能力均无影响。另一方面，繁殖力随密度增加，每天有 1.66 个儿子/♀/天，与对照相似。我们得出结论，每笼 30000 头蛹（0.144 个寄生蜂/cm2）的密度适合大规模预释放包装寄生蜂 D. longicaudata。关于热休眠期，在 2±2°C 条件下 180 分钟，被认为是管理时间，不会影响成虫的存活率、繁殖力和飞行能力。获得的结果对于确定用于生物防治实蝇科水果蝇种群的 D. longicaudata 的预释放包装参数具有重要的实用价值。
