Wang Xuejing, Tian Liangfei, Ren Yongshuo, Zhao Zhongyang, Du Hang, Zhang Zhizhou, Drinkwater Bruce W, Mann Stephen, Han Xiaojun
State Key Laboratory of Urban Water Resource and Environment School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, China.
Faculty of Engineering, Queens Building, University of Bristol, Bristol, BS8 1TR, UK.
Small. 2020 Jul;16(27):e1906394. doi: 10.1002/smll.201906394. Epub 2020 Feb 27.
An ultrasound-based platform is established to prepare homogenous arrays of giant unilamellar vesicles (GUVs) or red blood cell (RBCs), or hybrid assemblies of GUV/RBCs. Due to different responses to the modulation of the acoustic standing wave pressure field between the GUVs and RBCs, various types of protocell/natural cell hybrid assemblies are prepared with the ability to undergo reversible dynamic reconfigurations from vertical to horizontal alignments, or from 1D to 2D arrangements. A two-step enzymatic cascade reaction between transmitter glucose oxidase-containing GUVs and peroxidase-active receiver RBCs is used to implement chemical signal transduction in the different hybrid micro-arrays. Taken together, the obtained results suggest that the ultrasound-based micro-array technology can be used as an alternative platform to explore chemical communication pathways between protocells and natural cells, providing new opportunities for bottom-up synthetic biology.
建立了一个基于超声的平台，用于制备巨型单层囊泡（GUVs）或红细胞（RBCs）的均匀阵列，或GUV/RBCs的混合组装体。由于GUVs和RBCs对声驻波压力场调制的响应不同，制备了各种类型的原细胞/天然细胞混合组装体，它们能够经历从垂直排列到水平排列，或从一维排列到二维排列的可逆动态重构。利用含葡萄糖氧化酶的发射体GUVs与过氧化物酶活性的受体RBCs之间的两步酶级联反应，在不同的混合微阵列中实现化学信号转导。综上所述，所得结果表明，基于超声的微阵列技术可作为探索原细胞与天然细胞之间化学通讯途径的替代平台，为自下而上的合成生物学提供新的机遇。
