Department of HIV/AIDS, National Institute for Research in Tuberculosis (Indian Council of Medical Research), Chennai, India.
YRG CARE Infectious Diseases Laboratory, YR Gaitonde Centre for AIDS Research and Education, Chennai, India.
AIDS Res Hum Retroviruses. 2020 Jun;36(6):533-538. doi: 10.1089/AID.2020.0027. Epub 2020 Apr 2.
In this study, we sequenced the full-length HIV type 2 (HIV-2) long terminal repeat region from the proviral DNA of 23 HIV-2-infected individuals from the southern parts of India. We identified two different promoter variant strains circulating in this region along with the globally circulating common promoter variant. Seven sequences had an additional nuclear factor kappa-light chain enhancer of activated B cells (NF-κB) binding motif and the sequence from another subject showed one NF-κB and one RBE-III binding site. Phylogenetic and subtyping analyses revealed that the circulating strains comprised HIV-2 subtype A. The occurrence of two NF-κB binding sites in ∼30% of the sequences analyzed in our study prompts us to hypothesize that as in the case of HIV-1 subtype C viruses that possess additional κB sites, the two NF-κB HIV-2 variants might possess superior replication fitness because of the increased magnitude of transcription, thus leading to the expansion of these variants in the country.
在这项研究中，我们对来自印度南部地区的 23 名 HIV-2 感染者的前病毒 DNA 进行了全长 HIV-2 长末端重复序列的测序。我们发现，除了在全球范围内流行的常见启动子变异株外，该地区还存在两种不同的启动子变异株。7 个序列具有额外的核因子κ轻链增强子的 B 细胞激活因子（NF-κB）结合基序，另一个序列显示了一个 NF-κB 和一个 RBE-III 结合位点。系统进化和亚型分析表明，流行的菌株包括 HIV-2 亚型 A。在我们研究中分析的约 30%的序列中出现了两个 NF-κB 结合位点，这促使我们假设，就像 HIV-1 亚型 C 病毒具有额外的 κB 位点一样，这两种 NF-κB HIV-2 变异株可能具有更高的复制适应性，因为转录的幅度增加，从而导致这些变异株在该国的扩张。
