State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Guizhou University, Guiyang 550025, China; Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang, 550025, China.
Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang, 550025, China.
Anal Chim Acta. 2020 Apr 1;1104:164-171. doi: 10.1016/j.aca.2020.01.007. Epub 2020 Jan 3.
This paper demonstrated a simple and validated fluorescence enhancing method to selectively recognize and discriminate the amino acid phenylalanine (Phe). H NMR spectroscopy reveal that the palmatine (PAL) can be encapsulated into the cucurbit [8]uril (Q [8]) in aqueous solution to form stable 1:2 host-guest inclusion complex PAL@Q [8], which exhibits moderate intensity fluorescence property. Interestingly, the addition of the Phe into the inclusion complex PAL@Q [8] leads to dramatically enhancing of the fluorescence intensity. In contrast, the addition of any other natural amino acids into the inclusion complex PAL@Q [8] gives no fluorescence variation. Furthermore, it is easy to detect the concentration of Phe in target aqueous solution according to the linear relationship between fluorescence intensity and concentration of the Phe.
本文展示了一种简单且经过验证的荧光增强方法，可选择性地识别和区分氨基酸苯丙氨酸（Phe）。H NMR 光谱表明，小檗碱（PAL）可以在水溶液中被包裹到葫芦[8]脲（Q[8]）中，形成稳定的 1:2 主客体包合物 PAL@Q[8]，具有中等强度的荧光性质。有趣的是，向包含物复合物 PAL@Q[8]中加入 Phe 会导致荧光强度显著增强。相比之下，向包含物复合物 PAL@Q[8]中加入任何其他天然氨基酸都不会引起荧光变化。此外，根据 Phe 的荧光强度与浓度之间的线性关系，很容易检测到目标水溶液中 Phe 的浓度。
