Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang 550025, China.
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jun 5;233:118177. doi: 10.1016/j.saa.2020.118177. Epub 2020 Feb 19.
Herein we describe a simple fluorescence quenching method for the selective recognition and determination of the amino acid phenylalanine (Phe). The use of H NMR spectroscopy revealed that the alkaloid palmatine (PAL) can encapsulated partially into the cavity of cucurbit[7]uril (Q[7]) in aqueous solution to form a stable 1:1 host-guest inclusion complex. This host-guest complex exhibits fluorescence of moderate intensity. Interestingly, the addition of the Phe results in a dramatic quenching of the fluorescence intensity associated with the inclusion complex. By contrast, the addition of other natural amino acids resulted in no change in the fluorescence. Based on the linear relationship between the fluorescence intensity and the concentration of Phe, the detection of the concentration of Phe in aqueous solution is facile. Thus, a new fluorescence quenching method for the recognition and determination of the Phe has established herein.
在这里，我们描述了一种简单的荧光猝灭方法，用于选择性识别和测定氨基酸苯丙氨酸（Phe）。使用 H NMR 光谱学表明，生物碱巴马汀（PAL）可以部分包裹在葫芦[7]脲（Q[7]）的空腔中，在水溶液中形成稳定的 1:1 主客体包合物。该主体客体配合物表现出中等强度的荧光。有趣的是，加入 Phe 会导致与包合物相关的荧光强度急剧猝灭。相比之下，加入其他天然氨基酸不会改变荧光。基于荧光强度与 Phe 浓度之间的线性关系，可以方便地检测水溶液中 Phe 的浓度。因此，本文建立了一种用于识别和测定 Phe 的新的荧光猝灭方法。
