Li W Q, Zhao W C, Xin J, Niu T L, Chao Y F, Zhou P, Zheng M H, Xu B
Department of Quality Control, Taizhou People's Hospital, Taizhou, Jiangsu, China.
Department of Urology, Taizhou People's Hospital, Taizhou, Jiangsu, China.
J Biol Regul Homeost Agents. 2020 Feb 28;34(1). doi: 10.23812/19-460-A.
Expression of microRNA(miR)-142-3p has been implicated to be associated with several cancers, whereas its function in bladder cancer (BC) remains unknown. The present study aimed to explore the correlation between the expression of miR-142-3p and the proliferation, migration and invasion of bladder cancer cells by activating Rac1. qRT-PCR was used to measure the expression of miR-142- 3p in bladder cancer tissues and cell lines. RNA transfection was used to silence and accelerate the expression of miR-142-3p in bladder cancer cells. CCK-8 and trans-well assays were used to detect the proliferation, migration and invasion of cells before and after RNA transfection. The direct interaction between Rac1 and miR-142-3p was demonstrated by a dual luciferase reporter assay. qRT-PCR and Western blot assays were used to detect the expression changes in Rac1 before and after transfection. The results showed that miR-142-3p in bladder cancer tissues was significantly lower than that in adjacent tissues and lower than that in HT1376 and T-24 cells but higher than that in T5637 and BIU- 87 cells. Additionally, upregulating miR-142-3p expression not only inhibits the proliferation of SV-HUC-1 and BIU-87 cells but also inhibits migration and invasion, and downregulating miR-142-3p expression showed the opposite results. The expression of Rac1 was promoted after stimulating miR- 142-3p expression, but was inhibited after silencing miR-142-3p expression. In conclusion, miR-142-3p affects the proliferation, migration and invasion of bladder cancer cells by regulating Rac1.
微小RNA（miR）-142-3p的表达已被证实与多种癌症相关，但其在膀胱癌（BC）中的功能尚不清楚。本研究旨在通过激活Rac1来探讨miR-142-3p的表达与膀胱癌细胞增殖、迁移和侵袭之间的相关性。采用qRT-PCR检测miR-142-3p在膀胱癌组织和细胞系中的表达。利用RNA转染来沉默和加速miR-142-3p在膀胱癌细胞中的表达。采用CCK-8和Trans-well实验检测RNA转染前后细胞的增殖、迁移和侵袭能力。通过双荧光素酶报告基因实验证实Rac1与miR-142-3p之间存在直接相互作用。采用qRT-PCR和蛋白质免疫印迹实验检测转染前后Rac1的表达变化。结果显示，膀胱癌组织中miR-142-3p的表达显著低于癌旁组织，低于HT1376和T-24细胞中的表达，但高于T5637和BIU-87细胞中的表达。此外，上调miR-142-3p的表达不仅抑制SV-HUC-1和BIU-87细胞的增殖，还抑制其迁移和侵袭，而下调miR-142-3p的表达则产生相反的结果。刺激miR-142-3p表达后Rac1的表达升高，而沉默miR-142-3p表达后Rac1的表达受到抑制。综上所述，miR-142-3p通过调节Rac1影响膀胱癌细胞的增殖、迁移和侵袭。
