Tripolyphosphate crosslinked (wheatgrass) functionalized antimicrobial chitosan: Ameliorating effect on physicochemical, mechanical, invitro cytocompatibility and cell migration properties.

Polymeric films for various biomedical applications require to be biocompatible and non- toxic. Chemical route of modifications for functionalization of the films for improved properties lead to undesirable effects for biological applications. Hence a natural way to enhancing their properties is by functionalizing them using plant extracts. This report investigates the synthesis of bioactive phytochemical loaded polymer using (wheatgrass) extract incorporated in tripolyphosphate crosslinked chitosan. Physical and mechanical properties of the extract functionalized crosslinked chitosan were analyzed and this showed significant changes in thickness, tensile strength and % elongation of the blend. The extract functionalized chitosan was characterized using Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM) and Energy dispersive spectroscopy (EDAX) confirming the interaction between the functional moieties of the extract and polymer. Antimicrobial analysis showed improved activity against and and . Presence of the extract in crosslinked chitosan enhanced the cytocompatibility in 3T3 cells carried out by MTT assay and showed improved cell migration properties determined by scratch assay.Communicated by Ramaswamy H. Sarma.