Department of Otolaryngology-Head and Neck Surgery, Hunan Provincial Key Lab, Otolaryngology Institute of Major Diseases, The Xiangya Hospital, Central South University, Changsha, Hunan, China.
Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Acta Otolaryngol. 2020 May;140(5):351-355. doi: 10.1080/00016489.2020.1717609. Epub 2020 Feb 28.
Regarded as the most important clinical characteristic of middle ear cholesteatoma, the exact mechanism of bone resorption in cholesteatoma still remains unknown. To investigate protein expression of PTHrP and RANKL in acquired middle ear cholesteatoma epithelium and analyze their functional roles in the etiopathogenesis of bone resorption in middle ear cholesteatoma. A total of 22 patients who underwent surgical treatment for middle ear cholesteatoma were recruited in the study. Protein expression of PTHrP and RANKL in middle ear cholesteatoma and normal postauricular skin was investigated by immunohistochemical staining. Correlations between bone resorption degree and expression of PTHrP and RANKL were also analyzed. Protein expression of PTHrP and RANKL in cholesteatoma epithelium significantly increased when compared with normal postauricular skin epithelium. In cholesteatoma epithelium, a significantly positive association was observed between PTHrP and RANKL expression. Meanwhile, obviously positive correlations between protein expression of PTHrP and RANKL and bone resorption degree were discovered. The increased protein expression of PTHrP and RANKL in cholesteatoma epithelium, and their associations with the degree of bone resorption, revealing that PTHrP might promote bone resorption process in middle ear cholesteatoma through RANKL signaling pathway.
被认为是中耳胆脂瘤最重要的临床特征，胆脂瘤中确切的骨质吸收机制仍不清楚。本研究旨在探讨甲状旁腺激素相关蛋白（PTHrP）和核因子-κB 受体活化因子配体（RANKL）在获得性中耳胆脂瘤上皮中的蛋白表达，并分析它们在中耳胆脂瘤骨质吸收发病机制中的功能作用。共招募 22 例接受中耳胆脂瘤手术治疗的患者作为研究对象。采用免疫组织化学染色法检测中耳胆脂瘤和正常耳后皮肤中 PTHrP 和 RANKL 的蛋白表达，并分析骨吸收程度与 PTHrP 和 RANKL 表达的相关性。与正常耳后皮肤上皮相比，胆脂瘤上皮中 PTHrP 和 RANKL 的蛋白表达明显增加。在胆脂瘤上皮中，PTHrP 和 RANKL 的表达呈显著正相关。同时，还发现 PTHrP 和 RANKL 的蛋白表达与骨吸收程度之间存在明显的正相关。胆脂瘤上皮中 PTHrP 和 RANKL 的蛋白表达增加，且与骨吸收程度相关，表明 PTHrP 可能通过 RANKL 信号通路促进中耳胆脂瘤中的骨吸收过程。
