Opt Lett. 2020 Mar 1;45(5):1063-1066. doi: 10.1364/OL.385072.
Brillouin imaging is an emerging optical elastography technique that is able to generate maps of the mechanical properties at microscale with great potential in biophysical and biomedical fields. A key parameter is its spatial resolution, which is usually identified with that of the confocal microscope coupled to the Brillouin interferometer. Conversely, here we demonstrate that the mean free path of acoustic phonons plays a major role in defining the resolution, especially for high numerical aperture confocal setups. Surprisingly, the resolution of elastography maps may even deteriorate when decreasing the scattering volume.
布里渊成像是一种新兴的光学弹性成像技术，能够以微尺度生成机械性能图，在生物物理和生物医学领域具有巨大的潜力。一个关键参数是其空间分辨率，通常与耦合到布里渊干涉仪的共焦显微镜的分辨率一致。相反，在这里我们证明声子的平均自由程在确定分辨率方面起着主要作用，特别是对于高数值孔径共焦设置。令人惊讶的是，当减小散射体积时，弹性成像图的分辨率甚至可能恶化。
