Department of Marine Life Science, Jeju National University, Jeju, 63243, Republic of Korea.
Naturetech Co., 29-8, Yongjeong-gil, chopyeong-myeon, Jincheon-gun, Chungbuk, Republic of Korea.
Food Funct. 2020 Mar 26;11(3):2299-2308. doi: 10.1039/c9fo02924a.
This study aimed to demonstrate the anti-obesity effect of Plocamium telfairiae (PT), a red seaweed. Different percentages of ethanol (0%, 20%, 40%, 60%, 80%, and 100%) were used for the preparation of PT extract. Furthermore, 3T3-L1 cells were used to determine the percentage of ethanol for optimal anti-adipogenesis of PT, and the anti-obesity properties of the optimized extract of PT (PTE) (40%) was assessed in obese mice. The results indicate that 40% ethanol extract (40 PTE) significantly decreased fat accumulation and suppressed the expression of major adipogenesis factors such as peroxisome proliferator-activated receptor-γ (PPAR-γ), sterol regulatory element-binding protein 1 (SREBP-1), CCAAT/enhancer-binding protein (C/EBP)-α, and phosphorylated ACC (pACC) in 3T3-L1 cells. Furthermore, in the high-fat diet-induced obese mice, 40 PTE significantly reduced the weights of white adipose tissue, as well as the levels of triglyceride, total cholesterol, adiponectin, and insulin in the serum. Liver histopathology showed that steatosis decreased in all the PTE treatment groups. The adipogenesis-related proteins, PPAR-γ and SREBP-1, were also significantly decreased in PTE treatment groups. Additionally, 40 PTE increased mRNA expression of mitochondrial uncoupling proteins (UCP)-1 and UCP-3 in brown adipose tissue. These findings provide evidence that 40 PTE can alleviate lipid droplet accumulation in 3T3-L1 adipocytes and obese C57BL/6 mice, indicating that PTE has strong anti-obesity effects and could be used as a therapeutic agent or a component of pharmaceutical drugs and functional foods.
本研究旨在证明 Plocamium telfairiae（PT），一种红色海藻的抗肥胖作用。使用不同百分比的乙醇（0％，20％，40％，60％，80％和 100％）来制备 PT 提取物。此外，使用 3T3-L1 细胞来确定用于 PT 最佳抗脂肪生成的乙醇百分比，并且评估了 PT 的优化提取物（PTE）（40％）在肥胖小鼠中的抗肥胖特性。结果表明，40％乙醇提取物（40 PTE）可显著减少脂肪积累，并抑制主要脂肪生成因子的表达，如过氧化物酶体增殖物激活受体-γ（PPAR-γ），固醇调节元件结合蛋白 1（SREBP-1），CCAAT /增强子结合蛋白（C / EBP）-α和磷酸化 ACC（pACC）在 3T3-L1 细胞中。此外，在高脂肪饮食诱导的肥胖小鼠中，40 PTE 显著降低了白色脂肪组织的重量，以及血清中甘油三酯，总胆固醇，脂联素和胰岛素的水平。肝组织病理学显示，所有 PTE 治疗组的脂肪变性均减少。PPAR-γ和 SREBP-1 的脂肪生成相关蛋白也在 PTE 治疗组中明显降低。此外，40 PTE 增加了棕色脂肪组织中线粒体解偶联蛋白（UCP）-1 和 UCP-3 的 mRNA 表达。这些发现提供了证据，表明 40 PTE 可以减轻 3T3-L1 脂肪细胞和肥胖 C57BL / 6 小鼠中的脂质滴积累，表明 PTE 具有很强的抗肥胖作用，可作为治疗剂或药物和功能性食品的成分。
