Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai, P.R. China.
Department of Urology, Beijing Friendship Hospital, Capital Medical University of Tongzhou District, Beijing, P.R. China.
Cancer Biother Radiopharm. 2020 May;35(4):301-306. doi: 10.1089/cbr.2019.3319. Epub 2020 Feb 28.
Zhang et al. characterized a novel oncogenic long noncoding RNA (lncRNA) named NR2F2-AS1 in lung cancer. In this study, the role of lncRNA NR2F2-AS1 in clear cell renal cell carcinoma (ccRCC) was explored. Levels of NR2F2-AS1 and Rac1 mRNA expression in both cancer and noncancer tissues from 60 patients with ccRCC were measured by performing RT-qPCR. NR2F2-AS1 siRNA silencing and overexpression experiments were performed to analyze the role of NR2F2-AS1 in regulating Rac1 expression. Cell stemness was analyzed by stemness assay. NR2F2-AS1 was upregulated in ccRCC, and high NR2F2-AS1 expression levels in ccRCC tissues were associated with poor survival. Rac1 was also upregulated in ccRCC and positively correlated with NR2F2-AS1. In ccRCC cells, NR2F2-AS1 overexpression mediated the upregulation of Rac1, whereas NR2F2-AS1 siRNA was accompanied by Rac1 downregulation. NR2F2-AS1 and Rac1 overexpression resulted in the increased ccRCC cell stemness, whereas NR2F2-AS1 and Rac1 siRNA silencing played an opposite role. Rac1 overexpression inhibited the role of NR2F2-AS1 siRNA silencing. NR2F2-AS1 may upregulate Rac1 to increase cancer stemness in ccRCC.
Zhang 等人在肺癌中鉴定了一种新型致癌长非编码 RNA（lncRNA）NR2F2-AS1。在这项研究中，探索了 lncRNA NR2F2-AS1 在透明细胞肾细胞癌（ccRCC）中的作用。通过 RT-qPCR 测量了 60 例 ccRCC 患者癌组织和非癌组织中 NR2F2-AS1 和 Rac1 mRNA 表达水平。通过 NR2F2-AS1 siRNA 沉默和过表达实验分析 NR2F2-AS1 调节 Rac1 表达的作用。通过干细胞测定分析细胞干性。NR2F2-AS1 在 ccRCC 中上调，ccRCC 组织中高 NR2F2-AS1 表达水平与预后不良相关。Rac1 在 ccRCC 中也上调，与 NR2F2-AS1 呈正相关。在 ccRCC 细胞中，NR2F2-AS1 过表达介导 Rac1 的上调，而 NR2F2-AS1 siRNA 则伴有 Rac1 的下调。NR2F2-AS1 和 Rac1 的过表达导致 ccRCC 细胞干性增加，而 NR2F2-AS1 和 Rac1 siRNA 沉默则起到相反的作用。Rac1 的过表达抑制了 NR2F2-AS1 siRNA 沉默的作用。NR2F2-AS1 可能通过上调 Rac1 增加 ccRCC 中的癌症干性。
