Department of Integrative Medicine, Children's Hospital of Fudan University, Shanghai, China.
Mol Reprod Dev. 2020 Apr;87(4):503-511. doi: 10.1002/mrd.23329. Epub 2020 Feb 28.
Neonatal exposure to bisphenol A (BPA) is hypothesized to advance pubertal development. However, the effects of neonatal BPA exposure on pubertal development has not been described. In this study, female Sprague-Dawley rats were exposed to 0.05, 0.5, 5, or 10 mg·kg ·day BPA, or corn oil vehicle alone from postnatal day 1 (PND1) to PND10 via subcutaneous injection. We evaluated day of vaginal opening (DVO), ovarian morphology, serum hormone concentrations, and hypothalamic expression of Gnrh1 and Kiss1 in female rats at PND35. DVO was significantly advanced in rats exposed to 5 and 10 mg·kg ·day BPA. Serum hormone concentrations increased as BPA dose increased. Additionally, hypothalamic Gnrh1 and Kiss1 expression were increased with BPA exposure; rats exposed to 10 mg·kg ·day BPA had significantly upregulated hypothalamic Gnrh1 and Kiss1 expressions in terms of both messenger RNA and protein levels. Our results suggest that exposure to a 10 mg·kg ·day dose of BPA might advance pubertal development significantly. In addition, within the range of 0 to 10 mg·kg ·day , neonatal exposure to BPA may affect pubertal development in a dose-dependent manner.
新生儿期暴露于双酚 A（BPA）被假设为会促进青春期发育。然而，新生儿期 BPA 暴露对青春期发育的影响尚未被描述。在这项研究中，雌性 Sprague-Dawley 大鼠从出生后第 1 天（PND1）至 PND10 通过皮下注射接受 0.05、0.5、5 或 10mg·kg·day BPA，或玉米油载体单独处理。我们在 PND35 时评估了雌性大鼠的阴道开口日（DVO）、卵巢形态、血清激素浓度以及下丘脑中 Gnrh1 和 Kiss1 的表达。暴露于 5 和 10mg·kg·day BPA 的大鼠的 DVO 显著提前。随着 BPA 剂量的增加，血清激素浓度增加。此外，下丘脑中 Gnrh1 和 Kiss1 的表达随着 BPA 暴露而增加；暴露于 10mg·kg·day BPA 的大鼠在下丘脑 Gnrh1 和 Kiss1 的信使 RNA 和蛋白水平上均表现出显著上调。我们的结果表明，暴露于 10mg·kg·day BPA 剂量可能会显著促进青春期发育。此外，在 0 至 10mg·kg·day 范围内，新生儿期 BPA 暴露可能以剂量依赖的方式影响青春期发育。
