Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.
Centre for Stem Cells and Regenerative Medicine, King's College London, Guy's Hospital, London, UK.
Cell Stem Cell. 2020 Mar 5;26(3):441-457.e7. doi: 10.1016/j.stem.2020.01.012. Epub 2020 Feb 27.
Skin homeostasis is orchestrated by dozens of cell types that together direct stem cell renewal, lineage commitment, and differentiation. Here, we use single-cell RNA sequencing and single-molecule RNA FISH to provide a systematic molecular atlas of full-thickness skin, determining gene expression profiles and spatial locations that define 56 cell types and states during hair growth and rest. These findings reveal how the outer root sheath (ORS) and inner hair follicle layers coordinate hair production. We found that the ORS is composed of two intermingling but transcriptionally distinct cell types with differing capacities for interactions with stromal cell types. Inner layer cells branch from transcriptionally uncommitted progenitors, and each lineage differentiation passes through an intermediate state. We also provide an online tool to explore this comprehensive skin cell atlas, including epithelial and stromal cells such as fibroblasts, vascular, and immune cells, to spur further discoveries in skin biology.
皮肤稳态由数十种细胞类型共同调控，这些细胞类型共同指导干细胞更新、谱系分化和分化。在这里，我们使用单细胞 RNA 测序和单分子 RNA FISH 为全层皮肤提供了一个系统的分子图谱，确定了在毛发生长和休息期间定义 56 种细胞类型和状态的基因表达谱和空间位置。这些发现揭示了外根鞘 (ORS) 和内毛囊层如何协调毛发生成。我们发现 ORS 由两种相互混合但转录上不同的细胞类型组成，它们与基质细胞类型的相互作用能力不同。内层细胞从转录上未分化的祖细胞分支而来，每个谱系分化都要经过一个中间状态。我们还提供了一个在线工具来探索这个全面的皮肤细胞图谱，包括上皮细胞和基质细胞，如成纤维细胞、血管和免疫细胞，以激发皮肤生物学的进一步发现。
