Department of Pharmacology, Federal University of Paraná, Curitiba, PR, Brazil.
Paranaense University, Umuarama, PR, Brazil.
J Ethnopharmacol. 2020 May 23;254:112709. doi: 10.1016/j.jep.2020.112709. Epub 2020 Feb 25.
Cyperus rotundus L. (Cyperaceae) is considered one of the most widely distributed plant species in the world, especially in tropical and subtropical regions. In addition, it is commonly used in India, China and Japan in traditional medicine to treat different diseases, including dermatitis and other skin disorders.
To investigate the topical anti-inflammatory activity of C. rotundus rhizome ethanolic extract in models of acute and chronic dermatitis.
Phytochemical analysis was carried out using High-performance liquid chromatography-ultraviolet detection (HPLC/UV) to determine the presence of quercetin and chlorogenic acid in C. rotundus extract. Topical anti-inflammmatory effects of C. rotundus extract were evaluated on arachidonic acid (AA) and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation in mice. Skin biopsies were collected and submitted to histological and enzymatic analysis to evaluate the C. rotundus effect in leukocyte migration into inflamed tissue. Antiproliferative activity of C. rotundus was confirmed by PCNA immunostained cell analysis. Systemic and possible adverse effects of topical treatment with C. rotundus were evaluated by the skin atrophy and same organ weights. In addition, the glucocorticoid receptor (GR) antagonist mifepristone was used to investigate possible GR-mediated mechanisms of action.
The phytochemical analysis show that C. rotundus ethanol extract contains 45 μg/g of chlorogenic acid. Topical treatment with C. rotundus extract reduced ear edema and cellular infiltrate in acute and chronic skin inflammation models. Moreover, mice topically treated with C. rotundus exhibited decrease in TPA-induced keratinocyte hyperproliferation. Relevantly, topical treatment with C. rotundus did not caused skin atrophy or changes in lymphoid organ weight. The anti-inflammatory effect of C. rotundus was not influenced by the GR antagonist.
The results here demonstrate for the first time the topical anti-inflammatory and antiproliferative efficacy of C. rotundus extract, suggesting that the extract could be a potential new therapeutic tool for the treatment of inflammatory skin disorders.
香附（莎草科）被认为是世界上分布最广泛的植物之一，尤其在热带和亚热带地区。此外，它在印度、中国和日本的传统医学中被广泛用于治疗各种疾病，包括皮炎和其他皮肤疾病。
研究香附根茎乙醇提取物对急性和慢性皮炎模型的局部抗炎活性。
采用高效液相色谱-紫外检测（HPLC/UV）对香附提取物中的槲皮素和绿原酸进行植物化学分析。通过对香附提取物在花生四烯酸（AA）和 12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的小鼠皮肤炎症中的局部抗炎作用进行评价，来研究香附提取物的抗炎作用。采集皮肤活检标本，进行组织学和酶分析，以评价香附提取物对白细胞向炎症组织迁移的作用。通过 PCNA 免疫染色细胞分析证实香附的抗增殖活性。通过皮肤萎缩和同一器官重量评估香附的全身和可能的不良反应。此外，还使用糖皮质激素受体（GR）拮抗剂米非司酮来研究可能的 GR 介导的作用机制。
植物化学分析表明，香附乙醇提取物含有 45μg/g 的绿原酸。香附提取物的局部治疗可减少急性和慢性皮肤炎症模型中的耳部水肿和细胞浸润。此外，香附局部治疗的小鼠 TPA 诱导的角质形成细胞过度增殖减少。相关地，香附的局部治疗不会导致皮肤萎缩或淋巴器官重量的变化。GR 拮抗剂对香附的抗炎作用没有影响。
本研究首次证明了香附提取物的局部抗炎和抗增殖作用，表明该提取物可能是治疗炎症性皮肤疾病的一种有潜力的新治疗工具。
