Liu Zhigang, Iqbal Mudassar, Zeng Zhibo, Lian Yuxi, Zheng Aifang, Zhao Mengmeng, Li Zixin, Wang Guangjun, Li Zhifei, Xie Jun
Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China; College of Life Science, Anqing Normal University, Anqing, 246011, PR China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China.
University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100, Pakistan; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China.
Microb Pathog. 2020 Feb 25;142:104101. doi: 10.1016/j.micpath.2020.104101.
The pond has a complex microbial ecosystem, including microorganisms in water and sediment, which plays an important role in the health of fish and water quality. The microbial community structure in the ponds can be easily affected by many factors. However, not much is known about the role of different aquaculture model and fish on the microbial community structure in ponds. The purpose of the study was to investigate the microbial diversity and composition of the ponds with different aquaculture model and fish by high-throughput sequencing. A total of 3835072 valid sequences were achieved from 60 samples. Additionally, 2064 and 1917 core OTUs were observed in water and sediment samples, respectively. Our results suggested that sediment samples have a higher abundance and diversity of microbial community than water samples. In all the samples, the four most dominant phyla were Proteobacteria, Cyanobacteria, Actinomycetes and Bacteroides. At the genus level, hgcI_clade and CL500-29_marine_group were the dominant bacteria shared by the water samples and sediment samples. In addition, more bacteria related to eutrophication were found in the group of BF, BC and HSB, which suggested that these ponds may have been eutrophicated. In conclusion, the present study revealed the differences in the structure and diversity of microbial communities in ponds with different aquaculture model and fish. Furthermore, changes in typical bacteria of the ponds contribute to detect water quality and prevent water eutrophication.
该池塘拥有一个复杂的微生物生态系统，包括水体和沉积物中的微生物，这对鱼类健康和水质起着重要作用。池塘中的微生物群落结构很容易受到多种因素的影响。然而，关于不同养殖模式和鱼类对池塘微生物群落结构的作用，我们了解得并不多。本研究的目的是通过高通量测序调查不同养殖模式和鱼类的池塘中的微生物多样性和组成。从60个样本中总共获得了3835072条有效序列。此外，在水体和沉积物样本中分别观察到2064个和1917个核心操作分类单元（OTU）。我们的结果表明，沉积物样本中的微生物群落丰度和多样性高于水体样本。在所有样本中，四个最主要的门是变形菌门、蓝细菌门、放线菌门和拟杆菌门。在属水平上，hgcI_clade和CL500 - 29_marine_group是水体样本和沉积物样本共有的优势细菌。此外，在BF、BC和HSB组中发现了更多与富营养化相关的细菌，这表明这些池塘可能已经发生了富营养化。总之，本研究揭示了不同养殖模式和鱼类的池塘中微生物群落结构和多样性的差异。此外，池塘中典型细菌的变化有助于检测水质和防止水体富营养化。
