Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China.
Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China.
Food Chem. 2020 Jul 1;317:126456. doi: 10.1016/j.foodchem.2020.126456. Epub 2020 Feb 20.
In recent years, gold nanoclusters (AuNCs) have received considerable attention as optical transducers in chemo/biosensors. Herein, a facile and efficient assay for NO has been successfully developed based on the fluorescence quenching of AuNCs co-modified by bovine serum albumin and 3-mercaptopropionic acid (BSA/MPA-AuNCs). In the presence of NO under acidic conditions, Fe can be readily oxidized and transformed to Fe, which can significantly suppress the fluorescence of BSA/MPA-AuNCs via non-radiative electron-transfer mechanism. The linear range and detection limit for this system were found to be 5-30 μM (r = 0.9975) and 0.7 μM, respectively. Other common anions and cations showed only very minor interference with the NO detection. Furthermore, the effectiveness of the proposed sensing strategy was validated by the demonstration of good performance in the determination of the amount of NO in ham samples, rendering it a powerful tool for the assessment of food security and water quality.
近年来，金纳米簇（AuNCs）作为化学/生物传感器中的光传感器受到了广泛关注。在此，基于牛血清白蛋白和 3-巯基丙酸共修饰的 AuNCs（BSA/MPA-AuNCs）的荧光猝灭，成功开发了一种用于检测 NO 的简便、高效的方法。在酸性条件下存在 NO 时，Fe 可以被轻易氧化并转化为 Fe，这可以通过非辐射电子转移机制显著抑制 BSA/MPA-AuNCs 的荧光。该系统的线性范围和检测限分别为 5-30 μM（r=0.9975）和 0.7 μM。其他常见的阴离子和阳离子对 NO 的检测仅有非常小的干扰。此外，通过在火腿样品中测定 NO 的含量验证了所提出的传感策略的有效性，这为评估食品安全和水质提供了有力的工具。
