Fazeli Bahare, Mirhosseini Ali, Hashemi Zahra, Taheri Hossein
Immunology Research Center, Inflammation and Inflammatory Diseases Division, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Vascular Independent Research and Education, European Foundation, Milan, Italy.
Int Med Case Rep J. 2020 Feb 18;13:33-40. doi: 10.2147/IMCRJ.S225839. eCollection 2020.
Until recently, the aetiology of Buerger's disease (BD) has been unknown. Although there is a close relationship between BD and smoking, it cannot explain the low prevalence of BD among smokers or the disease's geographical distribution. Infectious pathogens, such as Rickettsial infection, have also been suggested as the trigger of BD development, but this theory has neither been proven nor ruled out. The aim of this study was to evaluate the footprint of Rickettsial infection in tissue specimens obtained from amputees with Buerger's disease. Forty-nine tissue biopsies were obtained from three below-the-knee amputees who also had a diagnosis of BD according to Olin's criteria (between 14-21 biopsies for each patient). After extraction of DNA from the tissue samples, the existence of 16srRNA was evaluated using a PCR test. The sequence of PCR products was evaluated using Geneious 11.1.2 software and NCBI blast. The 16srRNA was found in 3 to 7 samples from each patient. The sequence of the PCR products had a 98% homology with Rickettsia Tabaci. The sequences of the three patients were aligned, and no difference was found in the sequence of 16srRNA amongst the patients. Rickettsia Tabaci is a pathogen that infects tobacco leaves. Thus, BD might be an infectious disease for which smoking could be the route of pathogen entry into the bloodstreams of the sufferers. However, further studies are highly recommended to confirm this hypothesis.
直到最近，伯格氏病（BD）的病因仍不明确。尽管BD与吸烟之间存在密切关系，但这无法解释吸烟者中BD的低患病率或该疾病的地理分布情况。诸如立克次体感染等传染性病原体也被认为是BD发病的触发因素，但这一理论既未得到证实，也未被排除。本研究的目的是评估从患有伯格氏病的截肢者组织标本中立克次体感染的痕迹。从三名膝下截肢者身上获取了49份组织活检样本，这些患者也根据奥林标准被诊断为BD（每位患者14 - 21份活检样本）。从组织样本中提取DNA后，使用PCR检测评估16srRNA的存在情况。使用Geneious 11.1.2软件和NCBI blast评估PCR产物的序列。在每位患者的3至7个样本中发现了16srRNA。PCR产物的序列与烟蓟马立克次体有98%的同源性。对三名患者的序列进行比对，患者之间16srRNA的序列未发现差异。烟蓟马立克次体是一种感染烟草叶片的病原体。因此，BD可能是一种传染病，吸烟可能是病原体进入患者血流的途径。然而，强烈建议进一步研究以证实这一假设。
