Toracchio Sonia, Caruso Rosario Alberto, Perconti Silvia, Rigoli Luciana, Betri Enrico, Neri Matteo, Verginelli Fabio, Mariani-Costantini Renato
Center for Advanced Studies (CAST/CeSI-MeT), "G. d'Annunzio" University of Chieti-Pescara, 66100 Chieti, Italy.
Department of Human Pathology in Adult and Developmental Age "Gaetano Barresi", Section of Pathology, University of Messina, 98123 Messina, Italy.
Microorganisms. 2020 Feb 26;8(3):324. doi: 10.3390/microorganisms8030324.
() is the major recognized risk factor for non-cardia gastric cancer (GC), but only a fraction of infected subjects develop GC, thus GC risk might reflect other genetic/environmental cofactors and/or differences in virulence among infectious strains. Focusing on a high GC risk area of Northern Italy (Cremona, Lombardy) and using archived paraffin-embedded biopsies, we investigated the associations between the and genotype variants and gastric intraepithelial neoplasia (GIN, 33 cases) versus non-neoplastic gastroduodenal lesions (NNGDLs, 37 cases). The gene and the and (s and m) genotypes were determined by polymerase chain reaction (PCR) and sequencing. was confirmed in 37/37 (100%) NNGDLs and detected in 9/33 GINs (27%), consistently with the well-known loss in GC. was detected in 4/9 -positive GINs and in 29/37 NNGDLs. The s1a and m1 subtypes were more common in GINs than in NNGDLs (6/7 vs. 12/34, p=0.014, for s1a; 7/7 vs. 18/34, p=0.020 for m1), with significant s genotype-specific variance. The GIN-associated s1a sequences clustered together, suggesting that aggressive strains from a unique founder contribute to GC in the high-risk area studied.
（）是公认的非贲门胃癌（GC）的主要风险因素，但只有一小部分感染者会患GC，因此GC风险可能反映了其他遗传/环境辅助因素和/或感染菌株之间毒力的差异。聚焦于意大利北部（伦巴第大区克雷莫纳）的一个高GC风险地区，并使用存档的石蜡包埋活检组织，我们研究了（）和（）基因型变异与胃上皮内瘤变（GIN，33例）和非肿瘤性胃十二指肠病变（NNGDLs，37例）之间的关联。通过聚合酶链反应（PCR）和测序确定（）基因以及（）和（）（s和m）基因型。在37/37（100%）例NNGDLs中证实存在（），在9/33例GINs中检测到（）（27%），这与GC中众所周知的（）缺失一致。在4/9例（）阳性GINs和29/37例NNGDLs中检测到（）。（）的s1a和m1亚型在GINs中比在NNGDLs中更常见（s1a为6/7 vs. 12/34，p = 0.014；m1为7/7 vs. 18/34，p = 0.020），具有显著的（）基因型特异性差异。与GIN相关的（）s1a序列聚集在一起，表明来自一个独特祖先的侵袭性（）菌株促成了所研究的高风险地区的GC。
