Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13/29, 10-718 Olsztyn, Poland.
Independent Public Health Care Center of the Ministry of the Interior and Administration, and the Warmia and Mazury Oncology Center in Olsztyn, Wojska Polskiego 37, 10-228 Olsztyn, Poland.
Toxins (Basel). 2020 Feb 26;12(3):144. doi: 10.3390/toxins12030144.
The objective of this study was to determine the effect of long-term (48 days), administration of specific zearalenone (ZEN) doses (20 and 40 μg ZEN/kg BW in experimental groups EI and EII, which were equivalent to 200% and 400% of the upper range limit of the no-observed-adverse-effect-level (NOAEL), respectively) on the bioavailability of ZEN and the rate of changes in estradiol and testosterone concentrations in the peripheral blood of pre-pubertal gilts. ZEN and α-ZEL levels were similar until day 28. After day 28, α-ZEL concentrations increased significantly in group EI, whereas a significant rise in ZEN levels was noted in group EII. The presence of estradiol in peripheral blood plasma was not observed until day 20 of the experiment. Spontaneous secretion of estradiol was minimal, and it was determined at very low levels of up to 10 pg/mL in EI and EII groups. Testosterone concentrations ranged from 4 to 9 ng/mL in all groups. A decrease in the concentrations of both analyzed hormones was reported in the last stage of the experiment. The results of the experiment indicate that: (i) The bioavailability of ZEN in peripheral blood has low diagnostic value, (ii) exposure to low doses of ZEN induces minor changes in the concentrations of the analyzed hormones, which could lead to situational supraphysiological hormone levels and changes in endogenous hormonal balance.
本研究旨在确定长期（48 天）给予特定玉米赤霉烯酮（ZEN）剂量（实验组 EI 和 EII 中分别为 20 和 40μg ZEN/kg BW，相当于无明显不良效应水平（NOAEL）上限范围的 200%和 400%）对 ZEN 生物利用度以及未成年母猪外周血雌二醇和睾酮浓度变化率的影响。ZEN 和α-ZEL 水平在第 28 天之前相似。第 28 天之后，EI 组α-ZEL 浓度显著升高，而 EII 组 ZEN 水平显著升高。在外周血血浆中直到实验第 20 天才观察到雌二醇的存在。雌二醇的自发分泌很少，在 EI 和 EII 组中仅检测到低至 10 pg/mL 的水平。所有组的睾酮浓度均在 4 至 9 ng/mL 之间。在实验的最后阶段报告了两种分析激素浓度的下降。实验结果表明：（i）外周血中 ZEN 的生物利用度具有较低的诊断价值，（ii）暴露于低剂量 ZEN 会引起分析激素浓度的微小变化，这可能导致情境性超生理激素水平和内源性激素平衡的改变。
