Farhadi Ramin, Mohammadi Ghanatghestani Amir Hossein, Abbasi Roughayeh, Farshidfar Dina, Najafi Abouzar
Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.
Department of Animal and Poultry Sciences, Faculty of Agricultural Technologies, University of Tehran, Tehran, Iran.
BMC Vet Res. 2025 Jun 9;21(1):407. doi: 10.1186/s12917-025-04852-3.
This study aimed to investigate the protective role of different concentrations of lavender ethanolic extract (0, 75, 150, and 300 μg/dL) on the microscopic, oxidative stress, structural, and fertility parameters of ram epididymal sperm after the freezing/thawing process. Post-thaw evaluations included motility parameters, membrane integrity, acrosome membrane integrity, abnormality, MDA concentration, superoxide dismutase and glutathione peroxidase enzyme activities, viability, apoptosis stages, mitochondrial membrane potential, DNA fragmentation, ROS levels, and fertility parameters (pregnancy rate, birth rate, and lambing rate). The results indicated that the extender containing 150 µg/dL lavender extract improved motility parameters, including progressive motility and VCL compared to the control extender. In the evaluation of structural and biochemical parameters, the addition of 150 µg/dL lavender extract significantly enhanced plasma membrane and acrosome integrity, as well as superoxide dismutase activity, while reducing sperm abnormalities and MDA concentration compared to the control group. There was no significant difference in glutathione peroxidase activity between different extract concentrations. Furthermore, the presence of 150 µg/dL lavender extract increased mitochondrial membrane potential and sperm viability, while significantly decreasing HO free radical levels and DNA fragmentation, compared to the control group. In terms of fertility parameters, the use of an extender containing 150 µg/dL extract significantly increased pregnancy, birth, and lambing rates, although no significant difference was observed in the twinning rate between groups. In conclusion, adding 150 µg/dL lavender extract can improve the quality and fertility of ram semen after freezing/thawing, and its use is recommended.
本研究旨在探讨不同浓度薰衣草乙醇提取物（0、75、150和300μg/dL）对冻融后公羊附睾精子的微观、氧化应激、结构和生育参数的保护作用。解冻后评估包括活力参数、膜完整性、顶体膜完整性、异常率、丙二醛浓度、超氧化物歧化酶和谷胱甘肽过氧化物酶活性、存活率、凋亡阶段、线粒体膜电位、DNA片段化、活性氧水平和生育参数（妊娠率、出生率和产羔率）。结果表明，与对照稀释液相比，含有150μg/dL薰衣草提取物的稀释液改善了活力参数，包括前向运动和曲线速度。在结构和生化参数评估中，添加150μg/dL薰衣草提取物显著提高了质膜和顶体完整性以及超氧化物歧化酶活性，同时与对照组相比降低了精子异常率和丙二醛浓度。不同提取物浓度之间的谷胱甘肽过氧化物酶活性没有显著差异。此外，与对照组相比，150μg/dL薰衣草提取物的存在增加了线粒体膜电位和精子存活率，同时显著降低了羟基自由基水平和DNA片段化。在生育参数方面，使用含有150μg/dL提取物的稀释液显著提高了妊娠率、出生率和产羔率，尽管各组之间的双羔率没有显著差异。总之，添加150μg/dL薰衣草提取物可以提高冻融后公羊精液的质量和生育能力，建议使用。
