Department of Surgery, Show Chwan Memorial Hospital, Changhua, Taiwan, R.O.C.
Emergency Department, Cathay General Hospital, Taipei, Taiwan, R.O.C.
In Vivo. 2020 Mar-Apr;34(2):631-638. doi: 10.21873/invivo.11817.
BACKGROUND/AIM: Hepatocellular carcinoma (HCC) is a primary malignancy of the liver and the third leading cause of cancer death worldwide. Although multiple chemotherapies options are available for HCC, chemo-induced toxicity is inevitable during clinical treatment. Therefore, identifying possible adjuvant agents with both liver-protective and antitumor effects is critical. Herbal medicines have chemopreventive and anti-HCC effect, such as Juzen taiho-to and Sho-saiko-to. Astragaloside IV is a compound extracted from the Chinese medical herb Astragalus membranaceus (Fisch.) Bge. with liver protection potential. However, whether astragaloside IV may also possess tumor-inhibitory capability and its underlying mechanism is remaining unknown.
Viability analysis, cell-cycle analysis, apoptosis analysis, western blotting analysis and invasion trans-well assay were performed to identify tumor-inhibitory potential of astragaloside IV on HCC cells (SK-Hep1 and Hep3B cells).
We found that astragaloside IV may induce cytotoxicity and extrinsic/intrinsic apoptosis effect, but also trigger G arrest in HCC cells. The expression of anti-apoptotic proteins of HCC were all reduced by astragaloside IV. Additionally, astragaloside IV also suppressed HCC cell invasion ability.
Astragaloside IV effectively suppressed HCC cell proliferation, invasion and anti-apoptosis in vitro.
背景/目的：肝细胞癌（HCC）是肝脏的原发性恶性肿瘤，也是全球癌症死亡的第三大主要原因。尽管有多种化疗方案可用于 HCC，但在临床治疗中不可避免会发生化疗诱导的毒性。因此，寻找具有肝脏保护和抗肿瘤作用的可能辅助剂至关重要。草药具有化学预防和抗 HCC 作用，如 Juzen taiho-to 和 Sho-saiko-to。黄芪甲苷是从中药黄芪（Fisch.）Bge. 中提取的一种化合物，具有肝脏保护潜力。然而，黄芪甲苷是否也具有肿瘤抑制能力及其潜在机制尚不清楚。
通过活力分析、细胞周期分析、凋亡分析、Western blot 分析和侵袭 Trans-well 分析，鉴定黄芪甲苷对 HCC 细胞（SK-Hep1 和 Hep3B 细胞）的肿瘤抑制潜力。
我们发现黄芪甲苷可能诱导 HCC 细胞的细胞毒性和外源性/内源性细胞凋亡作用，同时触发 G 期细胞阻滞。黄芪甲苷还降低了 HCC 细胞中抗凋亡蛋白的表达。此外，黄芪甲苷还抑制了 HCC 细胞的侵袭能力。
黄芪甲苷在体外有效抑制 HCC 细胞的增殖、侵袭和抗凋亡。
