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Alexa Fluor 568 的热带反斯托克斯荧光特性。

Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.

机构信息

Department of Optics and Quantum Electronics, University of Szeged, Dóm tér 9., Szeged, 6720, Hungary.

出版信息

J Fluoresc. 2020 May;30(3):437-443. doi: 10.1007/s10895-020-02496-0. Epub 2020 Feb 29.

DOI:10.1007/s10895-020-02496-0
PMID:32112289
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7250809/
Abstract

Hot-band absorption and anti-Stokes emission properties of an organic fluorescent dye, Alexa Fluor 568, were characterized and compared with those of Rhodamine 101. The comparison of the properties (e.g., quantum efficiency, spectral distribution, thermal properties, and fluorescence lifetime) between the two dyes confirms that both dyes undergo the same process when excited in the red spectral region. Possible undesirable crosstalk effects and applications in dSTORM microscopy were demonstrated and discussed.

摘要

热带吸收和反斯托克斯发射特性的一种有机荧光染料，Alexa Fluor 568，并与罗丹明 101 的进行了比较。对这两种染料的性能（如量子效率、光谱分布、热性质和荧光寿命）进行比较，证实了这两种染料在激发到红色光谱区域时都经历相同的过程。可能存在的不良串扰效应及在 dSTORM 显微镜中的应用进行了演示和讨论。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd0/7250809/62c0d774b0b6/10895_2020_2496_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd0/7250809/c4ed166921e8/10895_2020_2496_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd0/7250809/460c72550f02/10895_2020_2496_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd0/7250809/82ac8e8c9db0/10895_2020_2496_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd0/7250809/e91e0d0af783/10895_2020_2496_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd0/7250809/62c0d774b0b6/10895_2020_2496_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd0/7250809/c4ed166921e8/10895_2020_2496_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd0/7250809/460c72550f02/10895_2020_2496_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd0/7250809/82ac8e8c9db0/10895_2020_2496_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd0/7250809/e91e0d0af783/10895_2020_2496_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdd0/7250809/62c0d774b0b6/10895_2020_2496_Fig5_HTML.jpg

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