Heshof Ruud, Visscher Bram, van de Zilver Eric, van de Vondervoort Rick, van Keulen Femke, Delahaije Roy J B M, Wind Richèle D
HAN BioCentre, HAN University of Applied Sciences, Laan van Scheut 2, 6525 EM, Nijmegen, the Netherlands.
AMB Express. 2020 Feb 28;10(1):41. doi: 10.1186/s13568-020-00974-z.
Due to the depletion of fossil fuel resources and concern about increasing atmospheric CO levels, the production of microbial oil as source for energy and chemicals is considered as a sustainable alternative. A promising candidate strain for the production of microbial oil is the oleaginous yeast Schwanniomyces occidentalis CBS 2864. To compete with fossil resources, cultivation and processing of S. occidentalis requires improvement. Currently, different cell wall disruption techniques based on mechanical, chemical, physiological, and biological methods are being investigated using a variety of oil producing yeasts and microalgae. Most of these techniques are not suitable for upscaling because they are technically or energetically unfavorable. Therefore, new techniques have to be developed to overcome this challenge. Here, we demonstrate an effective mild enzymatic approach for cell disruption to facilitate lipid extraction from the oleaginous yeast S. occidentalis. Most oil was released by applying 187 mg L tailor-made enzymes from Trichoderma harzianum CBS 146429 against the yeast cell wall of S. occidentalis at pH 5.0 and 40 °C with 4 h of incubation time after applying 1 M NaOH as a pretreatment step.
由于化石燃料资源的枯竭以及对大气中二氧化碳水平上升的担忧，微生物油作为能源和化学品来源的生产被视为一种可持续的替代方案。产油酵母西方许旺酵母CBS 2864是生产微生物油的一种有潜力的候选菌株。为了与化石资源竞争，西方许旺酵母的培养和加工需要改进。目前，正在使用各种产油酵母和微藻研究基于机械、化学、生理和生物学方法的不同细胞壁破坏技术。这些技术大多不适合扩大规模，因为它们在技术上或能量上都不利。因此，必须开发新技术来克服这一挑战。在这里，我们展示了一种有效的温和酶法用于细胞破碎，以促进从产油酵母西方许旺酵母中提取脂质。在作为预处理步骤施加1 M NaOH后，在pH 5.0和40°C下，用来自哈茨木霉CBS 146429的187 mg/L特制酶作用于西方许旺酵母的细胞壁，并孵育4小时，大部分油脂被释放出来。
