HAN BioCentre, University of Applied Sciences, P.O. Box 6960, 6503 GL Nijmegen, The Netherlands; Bioprocess Engineering, Wageningen University and Research, P.O. Box 16, 6700 AA Wageningen, The Netherlands.
HAN BioCentre, University of Applied Sciences, P.O. Box 6960, 6503 GL Nijmegen, The Netherlands.
Bioresour Technol. 2019 Oct;289:121672. doi: 10.1016/j.biortech.2019.121672. Epub 2019 Jun 20.
The oleaginous yeast Schwanniomyces occidentalis was previously isolated because of its excellent suitability to convert lignocellulosic hydrolysates into triacyl glycerides: it is able to use a broad range of sugars and is able to tolerate high concentrations of lignocellulosic hydrolysate inhibitors. Compared to other oleaginous yeasts S. occidentalis however produces a low content of unsaturated fatty acids. We show here that the linoleic acid content can be significantly improved by (over)expression Δ12-desaturases derived from S. occidentalis and Fusarium moniliforme. Expression was stable for the homologous expression but decreased during heterologous expression. Both homologous and heterologous expression of mCherry-Δ12-desaturase led to a 4-fold increase in linoleic acid from 0.02 g/g biomass to 0.08 g/g biomass resulting in the production of 2.23 g/L and 2.05 g/L of linoleic acid.
产油酵母 Schwanniomyces occidentalis 以前因其非常适合将木质纤维素水解物转化为三酰基甘油而被分离出来：它能够使用广泛的糖，并能够耐受高浓度的木质纤维素水解物抑制剂。然而，与其他产油酵母相比，S. occidentalis 产生的不饱和脂肪酸含量较低。我们在这里表明，通过（过度）表达来自 S. occidentalis 和串珠镰刀菌的 Δ12-脱饱和酶，可以显著提高亚油酸含量。同源表达的表达是稳定的，但在异源表达期间下降。mCherry-Δ12-脱饱和酶的同源和异源表达均导致亚油酸从 0.02 g/g 生物质增加 4 倍，达到 0.08 g/g 生物质，从而产生 2.23 g/L 和 2.05 g/L 的亚油酸。
