金纳米颗粒作为干细胞示踪的计算机断层扫描标志物。

Gold Nanoparticles as a Computed Tomography Marker for Stem Cell Tracking.

机构信息

Department of Biomedical Engineering, Institute for Quantitative Health Science and Engineering, Michigan State University, East Lansing, MI, USA.

出版信息

Methods Mol Biol. 2020;2126:155-166. doi: 10.1007/978-1-0716-0364-2_14.

DOI:10.1007/978-1-0716-0364-2_14

PMID:32112387

Abstract

Stem cell tracking is an essential prerequisite for effective stem cell therapy. Computed tomography (CT) imaging technique is an emerging quantitative tool to detect real time distribution of transplanted cells. Most of CT labels based on the high atomic number (Z) materials have concern over biocompatibility. The present book chapter describes a protocol for the use of biocompatible gold nanoparticles as a CT marker for efficient labeling of mesenchymal stem cells (MSCs) and subsequent cell tracking in rodent models.

摘要

干细胞示踪是有效干细胞治疗的必要前提。计算机断层扫描（CT）成像技术是一种新兴的定量工具，可用于实时检测移植细胞的分布。大多数基于高原子序数（Z）材料的 CT 标记物都存在对生物相容性的担忧。本章描述了一种使用生物相容性金纳米粒子作为间充质干细胞（MSCs）的 CT 标记物的方案，以有效标记细胞并随后在啮齿动物模型中进行细胞跟踪。