Sincrotrone Trieste S.C.p.A, Science Park, Trieste, Italy.
Nanomedicine. 2011 Oct;7(5):647-54. doi: 10.1016/j.nano.2011.01.010. Epub 2011 Feb 17.
The ability to track cells in small-animal models of human disease is important because it gives the potential to improve our understanding of the processes of disease progression as well as our understanding of the therapeutic effects of interventions. In this study gold nanoparticles have been used as a permanent marker of implanted normal and malignant cell grafts in combination with a suitable x-ray apparatus. Using x-ray computed tomography the micrometric three-dimensional distribution of these marked cells could be displayed with penetration depth, high cell sensitivity and high spatial resolution in rodent models of human diseases. In principle the method allows quantification of cell numbers at any anatomical location over time in small animals.
在人类疾病的小动物模型中追踪细胞的能力很重要，因为它有可能提高我们对疾病进展过程的理解，以及对干预治疗效果的理解。在这项研究中，金纳米颗粒已被用作植入的正常和恶性细胞移植物的永久标记物，与合适的 X 射线设备结合使用。使用 X 射线计算机断层扫描，可以在人类疾病的啮齿动物模型中以高细胞灵敏度和高空间分辨率显示这些标记细胞的微米级三维分布，穿透深度高。原则上，该方法允许在小动物的任何解剖位置随时间定量细胞数量。
