Entomology Section, Central Cotton Research Institute, Multan, Pakistan.
Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
Pest Manag Sci. 2020 Aug;76(8):2645-2652. doi: 10.1002/ps.5806. Epub 2020 Mar 11.
Imidacloprid, a neonicotinoid insecticide, is widely sprayed alone or mixed with other insecticides against Dysdercus koenigii, a potential pest of cotton in Pakistan. Recently, resistance to imidacloprid in field populations of D. koenigii has developed because of its overuse. Herein, we have investigated inheritance of imidacloprid resistance in D. koenigii and its cross-resistance to other insecticides.
The imidacloprid-selected population had a 91 421-fold increase in resistance to imidacloprid after six generations. Overlapping 95% fiducial limits of LC of the F (Imida-Sel ♂ × Sus-ST ♀) and F (Imida-Sel ♀ × Sus-ST ♂) suggested an autosomal and incomplete dominant resistance to imidacloprid (D = 0.84 for F and 0.86 for F ). Reciprocal backcrosses of the F and F with Sus-ST predicted a polygenic inheritance. Realized heritability of imidacloprid resistance was 0.38. When mean slope = 1.74 and h = 0.38, then 3-13 generations would be required for a ten-fold increase in LC at 90-20% intensity of selection. Very high cross-resistance to emamectin benzoate (143-fold), deltamethrin (1675-fold) and lambda-cyhalothrin (140-fold), and moderate cross-resistance to acetamiprid (37-fold) in the imidacloprid-selected strain compared to the field population were observed.
Imidacloprid resistance developed very quickly under continuous selection pressure in the laboratory. These factors might lead to an increasing likelihood of resistance development in field populations, if imidacloprid is used continuously without insecticide rotation for prolonged periods. The present results would be supportive for better management of D. koenigii by devising an effective resistance management strategy. © 2020 Society of Chemical Industry.
吡虫啉是一种新烟碱类杀虫剂，广泛用于防治巴基斯坦棉花潜在害虫棉红蝽，单独使用或与其他杀虫剂混合使用。由于其过度使用，田间种群对吡虫啉的抗性已发展。本文研究了棉红蝽对吡虫啉的抗性遗传及其对其他杀虫剂的交互抗性。
经过 6 代筛选，吡虫啉选择种群对吡虫啉的抗性增加了 91421 倍。F（吡虫啉选择♂×sus-ST♀）和 F（吡虫啉选择♀×sus-ST♂）的 LC 95%置信区间重叠表明，吡虫啉对棉红蝽呈常染色体不完全显性抗性（F 为 0.84，F 为 0.86）。F 和 F 与 sus-ST 的正反交预测了多基因遗传。吡虫啉抗性的现实遗传力为 0.38。当平均斜率=1.74，h=0.38 时，在选择强度为 90%-20%的情况下，LC 增加 10 倍需要 3-13 代。与田间种群相比，吡虫啉选择种群对甲维盐苯甲酸盐（143 倍）、溴氰菊酯（1675 倍）和高效氯氟氰菊酯（140 倍）表现出很高的交互抗性，对乙虫腈（37 倍）表现出中等交互抗性。
在实验室持续选择压力下，吡虫啉抗性迅速发展。如果连续长时间不轮换使用杀虫剂，而继续使用吡虫啉，这些因素可能会导致田间种群的抗性发展几率增加。本研究结果将为通过制定有效的抗性管理策略更好地管理棉红蝽提供支持。© 2020 英国化学学会。
