Department of Entomology, PMAS-Arid Agriculture University, Rawalpindi 44000, Pakistan.
Pesticides and Environmental Toxicology Laboratory, Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
Toxins (Basel). 2022 Oct 6;14(10):686. doi: 10.3390/toxins14100686.
Fabricius is a potential cotton and okra pest in South Asia. The realized heritability, risk assessment, and inheritance mode of Kurstaki (Btk) resistance were determined in the Dipel-selected (DIPEL-SEL) . The DIPEL-SEL strain had a 127.56-fold rise in Dipel resistance after nine generations compared to the laboratory reference strain (LAB-PK). The overlapping of 95% fiducial limits in the median lethal concentrations (LCs) of the F (DIPEL-SEL♂ × LAB-PK♀) and F (DIPEL-SEL♀ × LAB-PK♂) suggested a lack of sex linkage and an autosomal Dipel resistance. The dominance (D) values for the F (0.86) and F (0.94) indicated incompletely dominant resistance to Dipel. Backcrossing of the F♀ × Lab-PK♂ revealed a polygenic response of resistance to Dipel. The realized heritability estimation () of resistance to Dipel was 0.19. With 20% to 90% selection mortality, the generations required for a tenfold increase in LC of Dipel were 4.7-22.8, 3.1-14.9, and 2.3-11.1 at of 0.19, 0.29, and 0.39, respectively, and a constant slope of 1.56. At slope values of 2.56 and 3.56 with a constant = 0.19, 7.7-37.4 and 10.6-52.0 generations were needed to increase the tenfold LC of Dipel in the DIPEL-SEL . It is concluded that the DIPEL-SEL has an autosomal, incompletely dominant, and polygenic nature of resistance. The of 0.19 suggested that a high proportion of phenotypic variation for the Dipel resistance in was heritable genetic variation. The present results will support the creation of an effective and suitable resistance management plan for better control of .
法布里修斯是南亚地区棉花和秋葵的潜在害虫。在 Dipel 选择（DIPEL-SEL）中确定了库斯塔基（Btk）抗性的实现遗传力、风险评估和遗传模式。与实验室参考菌株（LAB-PK）相比，经过九代后，DIPEL-SEL 菌株对 Dipel 的抗性提高了 127.56 倍。F（DIPEL-SEL♂×LAB-PK♀）和 F（DIPEL-SEL♀×LAB-PK♂）的中致死浓度（LC）的 95%置信限重叠表明不存在性连锁，并且 Dipel 抗性是常染色体的。F（0.86）和 F（0.94）的显性（D）值表明对 Dipel 的不完全显性抗性。F♀×Lab-PK♂的回交显示出对 Dipel 抗性的多基因反应。对 Dipel 抗性的实现遗传力估计（）为 0.19。在 20%至 90%的选择死亡率下，LC 增加十倍所需的世代数分别为 4.7-22.8、3.1-14.9 和 2.3-11.1，在 = 0.19、0.29 和 0.39 时，斜率分别为 1.56。在斜率值为 2.56 和 3.56 且常数 = 0.19 时，需要 7.7-37.4 和 10.6-52.0 代才能增加 DIPEL-SEL 中 Dipel 的十倍 LC。结论是，DIPEL-SEL 具有常染色体、不完全显性和多基因抗性。0.19 的 值表明 Dipel 抗性在 中表型变异的很大一部分是可遗传的遗传变异。目前的结果将支持创建一个有效的和合适的抗性管理计划，以更好地控制。
