Department of Biochemistry, School of Life Sciences, Central University of Rajasthan, Bandarsindri, Kishangarh, 305817 Ajmer, Rajasthan, India.
Department of Microbiology, School of Life Sciences, Central University of Rajasthan, Bandarsindri, Kishangarh, 305817 Ajmer, Rajasthan, India.
Int J Biol Macromol. 2020 Jun 1;152:535-545. doi: 10.1016/j.ijbiomac.2020.02.297. Epub 2020 Feb 26.
Human immune cell toll-like receptors (TLRs) provide a novel chance for the development of the vaccine adjuvant engaging TLR signaling. A library of peptides was developed and peptides structure was generated through homology modeling and refinement. Further, these peptides were subjected to receptor-ligand interaction study against human immune cell TLRs using Schrödinger-suite software. Here, we identified the most potent ligands for each human immune cell receptor and identified it as a potent adjuvant. This work portrays the ability of binding of different known protein adjuvants with human TLRs 1--10. The significance of the study deals with the identification of adjuvant (ligand) for human TLRs individually which assist in the development of the optimal highly immunogenic vaccine.
人类免疫细胞 Toll 样受体 (TLR) 为开发与 TLR 信号转导相关的疫苗佐剂提供了新的机会。我们构建了一个肽文库，通过同源建模和精修生成了肽结构。此外，我们使用 Schrödinger-suite 软件针对人类免疫细胞 TLR 进行了受体-配体相互作用研究。在此，我们确定了每种人类免疫细胞受体的最有效配体，并将其鉴定为有效的佐剂。这项工作描述了不同已知蛋白佐剂与人类 TLR1-10 的结合能力。该研究的意义在于鉴定出可辅助开发最佳高免疫原性疫苗的人类 TLR 佐剂（配体）。
