Bastos Thiago Souza Azeredo, Faria Adriana Marques, Cavalcante Alliny Souza de Assis, Madrid Darling Melany de Carvalho, Zapa Dina Maria Beltrán, Nicaretta João Eduardo, Cruvinel Leonardo Bueno, Heller Luciana Maffini, Couto Luiz Fellipe Monteiro, Rodrigues Daniel de Castro, Ferreira Lorena Lopes, Soares Vando Edésio, Cadioli Fabiano Antônio, Lopes Welber Daniel Zanetti
Centro de Parasitologia Veterinária, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás - UFG, Goiânia, GO, Brazil.
Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais - UFMG, Belo Horizonte, MG, Brazil.
Exp Parasitol. 2020 Feb 21;211:107861. doi: 10.1016/j.exppara.2020.107861.
The aim of the present study was to compare the infection capacity of Trypanosoma vivax experimentally inoculated through different routes in calves naturally infected with latent Anaplasma marginale. On Day 0 of the study, 25 calves (breed: Girolando) were divided into five groups. The first four groups of five calves each received approximately 1 × 10 trypomastigotes of T. vivax through the intradermal, subcutaneous, intramuscular and intravenous routes. Another five animals remained unaffected to serve as A. marginale naturally infected controls. The study of T. vivax was performed on all calves from D+1 to D+30 using the Woo, Brener and blood smear techniques. PCR was performed on Days +1, +3, +4, +5, +28, +29 and + 30. The results indicated that T. vivax was capable of infecting and developing the disease in the calves independent of the inoculation route. A positive correlation was found between T. vivax and rectal temperature (P ≤ 0.05) and a negative correlation was seen between the protozoan and globular volume (P ≤ 0.05). Latent A. marginale in the calves acted as co-infection for T. vivax. Jaundice was seen only in calves with a high parasitemia by A. marginale. Therefore, in areas with the confirmed presence of T. vivax in bovines, this protozoan should be included in the complex denominated "Bovine Parasitic Sadness", which currently encompasses only Anaplasma and Babesia.
本研究的目的是比较在自然感染边缘无形体潜伏感染的犊牛中，通过不同途径实验接种的间日锥虫的感染能力。在研究的第0天，将25头犊牛（品种：吉罗兰多）分为五组。前四组每组五头犊牛，分别通过皮内、皮下、肌肉和静脉途径接种约1×10个间日锥虫的锥鞭毛体。另外五头动物未受影响，作为自然感染边缘无形体的对照。从D + 1至D + 30，使用Woo、Brener和血涂片技术对所有犊牛进行间日锥虫研究。在第+1、+3、+4、+5、+28、+29和+30天进行PCR。结果表明，间日锥虫能够在犊牛中感染并引发疾病，与接种途径无关。间日锥虫与直肠温度之间呈正相关（P≤0.05），与红细胞压积之间呈负相关（P≤0.05）。犊牛体内潜伏的边缘无形体作为间日锥虫的共感染因素。黄疸仅在边缘无形体导致高寄生虫血症的犊牛中出现。因此，在已确认存在牛间日锥虫的地区，这种原生动物应被纳入目前仅包括无形体和巴贝斯虫的“牛寄生虫病复合体”。 （注：原文中“globular volume”翻译为“红细胞压积”更符合医学语境，可能原文表述有误，推测应为“hematocrit”，但按照要求未做修改）
