National and Kapodistrian University of Athens, Department of Biology, Section of Ecology & Systematics, Panepistimioupoli, Athens GR-157 84, Greece.
National and Kapodistrian University of Athens, Department of Biology, Section of Ecology & Systematics, Panepistimioupoli, Athens GR-157 84, Greece; University of Bern, Faculty of Sciences, Institute of Cell Biology, Baltzerstrasse 4, CH-3012 Bern, Switzerland.
Sci Total Environ. 2020 Jun 1;719:137447. doi: 10.1016/j.scitotenv.2020.137447. Epub 2020 Feb 20.
A primary school was investigated for airborne fungi by a culture-based method, in classrooms underneath a green roof in comparison to conventional concrete roofs. A portable Burkard sampler was used for the collection of air samples onto petri dishes with 2% Malt Extract Agar. The fungal aerosol mean concentration was 71 CFU m (range 17-176 CFU m, median 51) in the classroom directly under the green roof, significantly lower than 192-228 CFU m (range 0-1090 CFU m, median 69) under the concrete roofs and 188-412 CFU m (range 0-2183 CFU m, median 771) in ground floor classrooms. The Indoor/Outdoor ratio was 0.4 for total fungi and 0.2-1.1 for predominant genera underneath the green roof, whereas 1-2.1 and 0.3-3.2 respectively for the rest of classrooms. The Potential Exposure Dose (PED) for fungal particles was calculated to 4.6 CFU kg and 9.3-35.3 CFU kg respectively. The genera Penicillium, Cladosporium and Aspergillus prevailed indoors and in ambient air. Aspergillus concentrations indoors correlated significantly with the concentration of the coarse fraction (PM10) of particulate matter. The genus Penicillium increased indoors during late spring and summer, in temperature 20-23 °C and relative humidity 42-53% and also predominated in ambient air, both indicative of multiple anthropogenic sources of amplification. The evidence about the green roof positive effect on microbial indoor air quality (mIAQ) is a matter of concern for further investigation.
一所小学的空气真菌采用培养法进行了调查，该小学的教室位于绿色屋顶之下，与传统的混凝土屋顶进行了对比。使用便携式 Burkard 采样器通过 2%麦芽提取物琼脂将空气样本收集到培养皿中。绿色屋顶正下方教室中的真菌气溶胶平均浓度为 71 CFU m（范围为 17-176 CFU m，中位数为 51），显著低于混凝土屋顶下的 192-228 CFU m（范围为 0-1090 CFU m，中位数为 69）和底层教室中的 188-412 CFU m（范围为 0-2183 CFU m，中位数为 771）。总真菌的室内/室外比值为 0.4，绿色屋顶下主要属的比值为 0.2-1.1，而其余教室的比值分别为 1-2.1 和 0.3-3.2。真菌颗粒的潜在暴露剂量（PED）分别计算为 4.6 CFU kg 和 9.3-35.3 CFU kg。青霉属、枝孢属和曲霉属在室内和环境空气中占优势。室内空气中的曲霉属浓度与颗粒物的粗颗粒（PM10）浓度显著相关。属青霉属在 20-23°C 的温度和 42-53%的相对湿度下，于春末和夏季在室内增加，并且在环境空气中也占主导地位，这都表明存在多种人为放大的来源。绿色屋顶对室内空气微生物质量（mIAQ）的积极影响的证据是进一步调查的关注问题。
