Basilico Maria de la Luz Z, Chiericatti Carolina, Aringoli E Elena, Althaus Rafael L, Basilico Juan Carlos
Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santiago del Estero 2829, (3000) Santa Fe. República Argentina.
Sci Total Environ. 2007 Apr 15;376(1-3):143-50. doi: 10.1016/j.scitotenv.2007.01.001. Epub 2007 Feb 23.
This study investigated concentration and types of airborne fungi spores of indoor air. Forty nine houses of Santa Fe city (Argentina) were examined during one year. This city is characterized by a warm climate with an annual mean temperature of 18.6 degrees C and a relative humidity of 74.6%. Based on similar characteristics, a group of representative houses were selected from both urban and suburban areas. The study began by evaluating the airborne fungal concentrations on environmental factors such as area (urban-suburban), season (winter-summer) and presence/absence of a convection gas-fired heating system during winter. Samples were taken with a Standard RCS centrifugal air sampler which operates on the principle of impact onto an agar media strip by centrifugal force. Strips were filled with malt extract agar containing chloramphenicol to inhibit bacterial growth. After incubation and identification, concentrations of airborne fungi were calculated as CFU/m(3). Indoor results showed the presence of thirteen dominant genera: Cladosporium (58.90%), Alternaria (8.68%), Epicoccum (5.74%), Fusarium (5.37%), Curvularia (3.50%), Acremonium (1.27%), Drechslera (1.26%), Penicillium (1.25%), Aspergillus (1.14%), Mucor (0.61%), Ulocladium (0.57%), Nigrospora (0.48%), Chrysosporium (0.42%) and yeast (3.74%), whose presence varied throughout the year. Multivariate Analyses of Variance were performed to study the influence of environmental factors on concentrations of fungal flora. The results obtained were significant for season (lambda=0.1225), area (lambda=0.6371) and for the presence of a convection gas-fired heating system during winter (lambda=0.4765). ANOVA test for the season showed the highest fungal levels (Geometric Mean) in the summer for Alternaria (181.97 CFU/m(3) vs. 17.38 CFU/m(3)), Fusarium (158.49 CFU/m(3) vs. 2.14 CFU/m(3)), Curvularia (66.07 CFU/m(3) vs. 1.62 CFU/m(3)), Acremonium (7.24 CFU/m(3) vs. 2.29 CFU/m(3)), Mucor (3.16 CFU/m(3) vs. 1.15 CFU/m(3)), Nigrospora (2.34 CFU/m(3) vs. 1.07 CFU/m(3)), Chrysosporium (2.73 CFU/m(3) vs. 1.23 CFU/m(3)). In winter, the highest levels (Geometric Mean) were for Penicillium (5.13 CFU/m(3) vs. 1.91 CFU/m(3)) and yeast (16.22 CFU/m(3) vs. 3.09 CFU/m(3)). As for the area, ANOVA showed the highest fungal levels (Geometric Mean) in suburban areas for Cladosporium (676.08 CFU/m(3) vs. 380.19 CFU/m(3)), Curvularia (6.76 CFU/m(3) vs. 4.27 CFU/m(3)) Ulocladium (3.31 CFU/m(3) vs. 1.20 CFU/m(3)) and yeast (18.62 CFU/m(3) vs. 4.90 CFU/m(3)), while Aspergillus (4.57 CFU/m(3) vs. 1.38 CFU/m(3)), showed the highest levels (Geometric Mean) in the urban area. On the other hand, only Cladosporium showed a higher level (Geometric Mean) in houses without convection gas-fired heating system during winter, compared to that corresponding to heated houses.
本研究调查了室内空气中空气传播真菌孢子的浓度和种类。在一年时间里,对阿根廷圣菲市的49所房屋进行了检测。该市气候温暖,年平均温度为18.6摄氏度,相对湿度为74.6%。基于相似特征,从城市和郊区选取了一组具有代表性的房屋。该研究首先评估了环境因素对空气中真菌浓度的影响,这些环境因素包括区域(城市 - 郊区)、季节(冬季 - 夏季)以及冬季是否存在对流燃气供暖系统。样本通过标准RCS离心空气采样器采集,该采样器基于离心力将空气冲击到琼脂培养基条上的原理进行操作。培养基条填充有含氯霉素的麦芽提取物琼脂,以抑制细菌生长。经过培养和鉴定后,空气中真菌的浓度以CFU/m³计算。室内检测结果显示存在13个优势属:枝孢属(58.90%)、链格孢属(8.68%)、附球菌属(5.74%)、镰刀菌属(5.37%)、弯孢属(3.50%)、顶孢霉属(1.27%)、德氏霉属(1.26%)、青霉属(1.25%)、曲霉属(1.14%)、毛霉属(0.61%)、格孢腔菌属(0.57%)、黑孢霉属(0.48%)、金孢子菌属(0.42%)和酵母(3.74%),它们的存在随全年变化。进行了多变量方差分析以研究环境因素对真菌菌群浓度的影响。所得结果对于季节(λ = 0.1225)、区域(λ = 0.6371)以及冬季对流燃气供暖系统的存在(λ = 0.4765)具有显著性。季节的方差分析测试表明,链格孢属(夏季181.97 CFU/m³ 对比冬季17.38 CFU/m³)、镰刀菌属(夏季158.49 CFU/m³ 对比冬季2.14 CFU/m³)、弯孢属(夏季66.07 CFU/m³ 对比冬季1.62 CFU/m³)、顶孢霉属(夏季7.24 CFU/m³ 对比冬季2.29 CFU/m³)、毛霉属(夏季3.16 CFU/m³ 对比冬季1.15 CFU/m³)、黑孢霉属(夏季2.34 CFU/m³ 对比冬季1.07 CFU/m³)、金孢子菌属(夏季2.73 CFU/m³ 对比冬季1.23 CFU/m³)在夏季的真菌水平(几何平均值)最高。在冬季,青霉属(5.13 CFU/m³ 对比1.91 CFU/m³)和酵母(16.22 CFU/m³ 对比3.09 CFU/m³)的水平(几何平均值)最高。至于区域,方差分析表明,枝孢属(郊区676.08 CFU/m³ 对比城市380.19 CFU/m³)、弯孢属(郊区6.76 CFU/m³ 对比城市4.27 CFU/m³)、格孢腔菌属(郊区3.31 CFU/m³ 对比城市1.20 CFU/m³)和酵母(郊区18.62 CFU/m³ 对比城市4.90 CFU/m³)在郊区的真菌水平(几何平均值)最高,而曲霉属(城市4.57 CFU/m³ 对比郊区1.38 CFU/m³)在城市的水平(几何平均值)最高。另一方面,与有供暖的房屋相比,仅枝孢属在冬季没有对流燃气供暖系统的房屋中显示出更高的水平(几何平均值)。