胰岛素样生长因子I和胰岛素样生长因子II基因在人胎儿及成人脑中以及神经胶质瘤中的表达。
The expression of insulin-like growth factor I and insulin-like growth factor II genes in the human fetal and adult brain and in glioma.
作者信息
Sandberg A C, Engberg C, Lake M, von Holst H, Sara V R
机构信息
Karolinska Institute's Department of Psychiatry, St. Göran's Hospital, Stockholm, Sweden.
出版信息
Neurosci Lett. 1988 Oct 31;93(1):114-9. doi: 10.1016/0304-3940(88)90022-5.
Expression of insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF-II) genes in the human brain and in glioma was studied by slot blot and Northern analysis following hybridization of IGF-I and IGF-II cDNA probes to isolated mRNA. In the fetal brain there were major IGF-I transcripts of 7.5 and 4.4 kilobases (kb) and one major IGF-II transcript of 6.0 kb. IGF-II mRNA was less abundant in the adult brain, with IGF-II mRNA being scarcely detectable. All glioma tissue examined displayed a marked enhancement of both IGF-I and IGF-II gene expression when compared to normal brain. IGF-I transcripts of 4.4 and 2.2-1.8 kb were identified. Similar to fetal brain a major IGF-II transcript of 6.0 kb was found in the glioblastoma examined.
通过将胰岛素样生长因子I（IGF-I）和胰岛素样生长因子II（IGF-II）cDNA探针与分离的mRNA杂交后进行狭缝印迹和Northern分析，研究了人脑中以及胶质瘤中IGF-I和IGF-II基因的表达情况。在胎儿脑中，存在7.5和4.4千碱基（kb）的主要IGF-I转录本以及一个6.0 kb的主要IGF-II转录本。IGF-II mRNA在成人大脑中含量较少，几乎检测不到。与正常脑相比，所有检测的胶质瘤组织均显示IGF-I和IGF-II基因表达显著增强。鉴定出4.4和2.2 - 1.8 kb的IGF-I转录本。与胎儿脑相似，在所检测的胶质母细胞瘤中发现了一个6.0 kb的主要IGF-II转录本。
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