一种流动注射化学发光法，利用鲁米诺-二高碘酸根合银(III)反应测定药物制剂中的视黄醇。
A Flow Injection Chemiluminescence Method for the Determination of Retinol in Pharmaceutical Formulations by Using Luminol-Diperiodatoargentate(III) Reaction.
作者信息
Asghar Muhammad, Ameen Fuad, Al-Nadhari Saleh, Waseem Amir, Yaqoob Mohammad, Alarfaj Abdullah A, Nabi Abdul
机构信息
Department of Chemistry, University of Balochistan.
Department of Botany & Microbiology, Faculty of Science, King Saud University.
出版信息
J Nutr Sci Vitaminol (Tokyo). 2020;66(1):10-18. doi: 10.3177/jnsv.66.10.
In this paper, a chemiluminescence (CL) method is proposed for retinol determination by combining flow injection (FI) methodology. The CL reaction is based on the oxidation of luminol by diperiodatoargentate(III) (DPA) in the presence of retinol. Under the optimum conditions, the relative CL intensity was linear to the concentration of retinol over the range 5.0×10-14 mg L (y=347.26x+2.5944, R=0.9999, n=8) with limit of detection (LOD) of 1.5×10 mg L (S/N=3) and limit of quantification (LOQ) of 5.0×10 mg L (S/N=10). The relative standard deviation (RSD) was from 1.04-3.4% over the range studied and injection throughputs of 150 h. The method was satisfactorily applied to retinol in pharmaceutical formulation samples. The samples were saponified and extracted with liquid-liquid extraction using ether as an extractant. The possible CL mechanism is supported by CL and UV-visible spectrophotometric studies.
本文提出了一种结合流动注射（FI）技术的化学发光（CL）法用于视黄醇的测定。该CL反应基于在视黄醇存在下，二高碘酸根合银（III）（DPA）氧化鲁米诺。在最佳条件下，相对CL强度在5.0×10⁻¹⁴ mg/L范围内与视黄醇浓度呈线性关系（y = 347.26x + 2.5944，R = 0.9999，n = 8），检测限（LOD）为1.5×10 mg/L（S/N = 3），定量限（LOQ）为5.0×10 mg/L（S/N = 10）。在所研究的范围内，相对标准偏差（RSD）为1.04 - 3.4%，进样通量为150 h⁻¹。该方法成功应用于药物制剂样品中视黄醇的测定。样品经皂化处理后，以乙醚为萃取剂进行液 - 液萃取。CL和紫外 - 可见分光光度研究支持了可能的CL机理。
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