Department of Chemistry, University of Balochistan, Sariab Road, Quetta-, 87300, Pakistan.
Anal Sci. 2020 Oct 10;36(10):1223-1227. doi: 10.2116/analsci.20P126. Epub 2020 May 22.
A novel method for the analysis of nalbuphine hydrochloride (NAL) is reported based on its enhancement effect on a diperiodatoargentate(III)-rhodamine-B (Ag(III) complex-Rh-B) chemiluminescence (CL) system in an aqueous sulfuric acid medium using flow-injection analysis (FIA). The optimal conditions of the CL reaction were: sulfuric acid, 10 M; Ag(III) complex, 2.0 × 10 M; Rh-B, 2.0 × 10 M; Brij-35, 0.01%; sample loop volume, 300 μL; and flow rate, 3.0 mL/min/stream. The limit of detection (LOD) and limit of quantification (LOQ) were 0.001 and 0.003 mg/L (S/N = 3 and 10); linear calibration range, 5 × 10 - 5.0 mg/L (R = 0.9999) and injection throughput, 150/h. The relative standard deviation (RSD) was from 0.8 - 3.2% over the range studied. The suggested technique was applied for the determination of NAL in pharmaceutical injections, compared with a reported spectrophotometric method, and obtained results were found to be satisfactory. Based on spectrophotometric studies, the most probable mechanism of the CL reaction has been briefly described and drawn schematically.
一种基于盐酸纳布啡（NAL）增强双过碘酸合银（III）-罗丹明 B（Ag（III）配合物-Rh-B）化学发光（CL）体系的分析新方法，在硫酸水溶液中通过流动注射分析（FIA）进行报告。CL 反应的最佳条件为：硫酸 10 M；Ag（III）配合物 2.0×10 M；Rh-B 2.0×10 M；Brij-35 0.01%；样品环体积 300 μL；流速 3.0 mL/min/流。检测限（LOD）和定量限（LOQ）分别为 0.001 和 0.003 mg/L（S/N = 3 和 10）；线性校准范围为 5×10 - 5.0 mg/L（R = 0.9999），进样速度为 150/h。在所研究的范围内，相对标准偏差（RSD）为 0.8-3.2%。该技术已应用于药物注射中 NAL 的测定，与报道的分光光度法相比，结果令人满意。基于分光光度研究，简要描述并示意性地绘制了 CL 反应的最可能机制。
