MSU-DOE Plant Research Lab, Michigan State University, East Lansing, Michigan, U.S.A.
Department of Plant Biology, Michigan State University, East Lansing, Michigan, U.S.A.
J Microsc. 2020 Jun;278(3):154-163. doi: 10.1111/jmi.12881. Epub 2020 Mar 20.
By being at the interface of the exocytic and endocytic pathways, the plant trans-Golgi network (TGN) is a multitasking and highly diversified organelle. Despite governing vital cellular processes, the TGN remains one of the most uncharacterized organelle of plant cells. In this review, we highlight recent studies that have contributed new insights and to the generation of markers needed to answer several important questions on the plant TGN. Several drugs specifically affecting proteins critical for the TGN functions have been extremely useful for the identification of mutants of the TGN in the pursuit to understand how the morphology and the function of this organelle are controlled. In addition to these chemical tools, we review emerging microscopy techniques that help visualize the TGN at an unpreceded resolution and appreciate the heterogeneity and dynamics of this organelle in plant cells.
植物的反式高尔基体网络（TGN）位于胞吐和胞吞途径的交界处，是一个多功能且高度多样化的细胞器。尽管它控制着重要的细胞过程，但 TGN 仍然是植物细胞中最未被充分描述的细胞器之一。在这篇综述中，我们强调了最近的研究，这些研究为回答关于植物 TGN 的几个重要问题提供了新的见解，并生成了所需的标记物。几种专门影响 TGN 功能关键蛋白的药物对于鉴定 TGN 的突变体非常有用，有助于了解这个细胞器的形态和功能是如何被控制的。除了这些化学工具外，我们还回顾了新兴的显微镜技术，这些技术有助于以前所未有的分辨率观察 TGN，并了解植物细胞中这个细胞器的异质性和动态性。
