Division of Molecular Hematology, Department of Laboratory Medicine, Lund Stem Cell Center, Faculty of Medicine, Lund University , Lund, Sweden.
RNA Biol. 2020 Aug;17(8):1214-1222. doi: 10.1080/15476286.2020.1732694. Epub 2020 Mar 1.
ABTRACT tRNA-derived fragments or tRFs were long considered merely degradation intermediates of full-length tRNAs; however, emerging research is highlighting unanticipated new and highly distinct functions in epigenetic control, metabolism, immune activity and stem cell fate commitment. Importantly, recent studies suggest that RNA epitranscriptomic modifications may provide an additional regulatory layer that dynamically directs tRF activity in stem and cancer cells. In this review, we explore current work illustrating unanticipated roles of tRFs in mammalian stem cells with a focus on the impact of post-transcriptional RNA modifications for the biogenesis and function of this growing class of small noncoding RNAs.
摘要 tRNA 衍生片段（tRFs）长期以来被认为仅仅是全长 tRNA 的降解中间体；然而，新出现的研究强调了在表观遗传控制、代谢、免疫活性和干细胞命运决定方面具有意想不到的全新且高度独特的功能。重要的是，最近的研究表明，RNA 表转录组修饰可能提供了一个额外的调节层，可在干细胞和癌细胞中动态指导 tRF 活性。在这篇综述中，我们探讨了目前说明 tRF 在哺乳动物干细胞中具有意想不到的作用的工作，重点介绍了转录后 RNA 修饰对这一不断增长的小非编码 RNA 类别的生物发生和功能的影响。
