Liu Shuxun, Laaksonen Oskar, Marsol-Vall Alexis, Zhu Baoqing, Yang Baoru
Food Chemistry and Food Development, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
Department of Food Science, College of Biological Sciences and Biotechnology, Beijing Key Laboratory of Forestry Food Processing and Safety, Beijing Forestry University, Beijing 100083, China.
J Agric Food Chem. 2020 Mar 18;68(11):3626-3637. doi: 10.1021/acs.jafc.0c01050. Epub 2020 Mar 10.
The profile of volatile compounds was investigated using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) during bilberry juice fermentation with nine non- yeasts, including , , , , , , , , and . Dynamic changes in volatile compounds were determined simultaneously with the development of ethanol concentration during fermentation. or produced more ethyl acetate than other yeast strains throughout fermentation, while fermentation with resulted in high accumulation of higher alcohols. was associated with high productions of pentane-2,3-dione, 3-hydroxybutan-2-one, 2-methylbutanal, and 3-methylbutanal. Among the 59 volatile compounds detected, generally, higher alcohols and monoterpenes accumulated constantly and reached the maximum concentration at the middle or later fermentation stage, whereas aldehydes, ketones, and acetals accumulated first followed by a significant drop. The production and accumulation dynamics of metabolites were highly dependent on the yeast species and the developing ethanol content.
在越橘汁与9种非酵母菌株(包括、、、、、、、和)发酵过程中,采用顶空固相微萃取结合气相色谱 - 质谱联用(HS - SPME - GC - MS)技术研究挥发性化合物的概况。在发酵过程中,随着乙醇浓度的变化,同时测定挥发性化合物的动态变化。在整个发酵过程中,或产生的乙酸乙酯比其他酵母菌株更多,而用发酵会导致高级醇的大量积累。与2,3 - 二酮戊烷、3 - 羟基 - 2 - 丁酮、2 - 甲基丁醛和3 - 甲基丁醛的高产量有关。在所检测到的59种挥发性化合物中,一般来说,高级醇和单萜类化合物不断积累,并在发酵中期或后期达到最大浓度,而醛类、酮类和缩醛类化合物先积累,随后显著下降。代谢产物的产生和积累动态高度依赖于酵母种类和不断增加的乙醇含量。