Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
Physiol Res. 2019 Dec 30;68(Suppl 4):S433-S443. doi: 10.33549/physiolres.934382.
Effects of low-frequency electromagnetic fields (LF EMF) on the activation of different tissue recovery processes have already been fully understood. Preliminary recent data demonstrated that a special case of sinusoidal electromagnetic fields, known as amplitude-modulated currents (AMC) could have a potential to accelerate the cell metabolism or cell migration. An AMC generator was designed to generate sinusoidal induced electric currents with the amplitude modulation and the harmonic carrier frequency of 5,000 Hz was modulated by frequencies of 1 to 100 Hz. The magnetic field peak was 6 mT, electric field intensity 2 V/m and the current density of induced electrical currents was approximately 1 A/m(2). The coil of the generator was adapted to easy handling and safe integration into the shelf of the CO(2) incubator. The shelf with the coil was prepared for the introduction of cells in standard plastic in vitro chambers. The tests focused on cells with migratory capacity after injury or during immunological processes and thus, mesenchymal stromal cells (MSC), dendritic cells (DC), and fibroblasts were chosen. The tests involved exposures of the cells to LF EMF (180 min/day) every day, for a period of three days, before examining them for cell death, morphology changes, and CD markers. The samples were tested by using MTT assay and the effects on the intracellular concentration of reactive oxygen species were quantified. The cell migration was finally measured with the help of the transwell migration assay. None of the cell types showed any decrease in the cell viability after the LF EMF application and the cells displayed minimum changes in reactive oxygen species. Functional changes (acceleration of cell migration) after AMC exposure were statistically significant for the MSC samples only. The acceleration of MSCs is associated with the production of MMP by these cells. The EMF has a potential to be a safe, clinically applicable selective activator of MSC homing, MSC paracrine production, and subsequent regeneration processes.
低频电磁场（LF EMF）对不同组织修复过程的激活作用已被充分理解。最近的初步数据表明，一种特殊形式的正弦电磁场，即调幅电流（AMC），可能具有加速细胞代谢或细胞迁移的潜力。设计了一种 AMC 发生器来产生具有幅度调制的正弦感应电流，其载波频率为 5000 Hz，调制频率为 1 至 100 Hz。磁场峰值为 6 mT，电场强度为 2 V/m，感应电流的电流密度约为 1 A/m(2)。发生器的线圈经过改良，便于操作，并可安全集成到 CO(2)培养箱的搁架中。装有线圈的搁架准备用于将细胞引入标准塑料体外培养室中。这些测试主要针对受伤或免疫过程中具有迁移能力的细胞，因此选择了间充质基质细胞（MSC）、树突状细胞（DC）和成纤维细胞。这些测试涉及将细胞暴露于 LF EMF（每天 180 分钟），持续三天，然后检查细胞死亡、形态变化和 CD 标记物。使用 MTT 测定法对样品进行测试，并定量测定细胞内活性氧物质的浓度。最后，借助 Transwell 迁移测定法测量细胞迁移。在 LF EMF 应用后，没有一种细胞类型的细胞活力出现下降，细胞内活性氧物质的变化最小。仅在 MSC 样本中，AMC 暴露后的功能变化（加速细胞迁移）具有统计学意义。MSC 的加速与这些细胞产生 MMP 有关。该电磁场有可能成为一种安全、临床适用的 MSC 归巢、MSC 旁分泌产生以及随后的再生过程的选择性激活剂。
